利用 Biacore T200 检测蛋白与蛋白结合的亲和力数据 KD。本实验利用 CM5 芯片偶联蛋白 A,蛋白 B 作为分析物检测结合的亲和力。蛋白 A 分子量为 24 kD,蛋白B分子量为 21 kD。关键词: 蛋白质,相互作用,SPR,Biacore
材料与试剂
仪器设备
实验步骤
一、仪器准备
二、配体偶联
三、样品检测过程
结果与分析
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