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产品说明书: KAPA Dual-Indexed Adapter Kit Illumina® PlatformsVendor   

适用说明:本说明书适用于KAPA Dual-Indexed Adapter Kit for Illumina platforms (08278555702) 和 the standalone KAPA Adapter Dilution Buffer (08278539001)。
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1)本文档中的页码,附录均指原说明书中的页码及附录;
2)本文档包含原说明书中的产品描述,产品应用及实验操作流程等主要信息,欲了解关于本产品详细说明及信息,建议参考原说明书 (点击面板上“下载PDF”可下载)。

Product Description

KAPA Dual-Indexed Adapter Kits for Illumina platforms are designed for use with KAPA DNA and RNA library preparation kits to construct libraries for sequencing on an Illumina sequencer. Each plate contains a set of 96 adapters, each with two, 8-nucleotide indexes (barcodes) for multiplexed sequencing applications.

The sequences of the sequencing indices (barcodes) included in KAPA Dual-Indexed Adapter Kits are given in Important Parameters: Index Sequences and Pooling Guidelines (p. 6). The highly dissimilar nature of indices minimizes the possibility of inaccurate index calling.

KAPA Adapter Dilution Buffer [10 mM Tris-HCl, (pH 8.0-8.5), 10 mM NaCl, 1 mM EDTA] is provided with the kit to ensure optimal performance when adapters require further dilution.

Product Applications

KAPA Dual-Indexed Adapters are used to uniquely label sequencing libraries generated from individual biological samples. This allows for the pooling of libraries prior to target capture or cluster generation, to enable multiplexed sequencing – which simplifies sample preparation and reduces the cost of next-generation sequencing for a wide range of applications.

Primary applications for the use of KAPA Dual-Indexed Adapter Kits for Illumina platforms include:
• Human whole genome sequencing, performed with an Illumina HiSeq X instrument
• whole exome or targeted sequencing, using Roche NimbleGen SeqCap EZ or IDT xGen Lockdown Probes or other hybridization capture systems, in combination with the appropriate blockers
• RNA-seq
• ChIP-seq
• other direct sequencing applications, e.g., microbial whole-genome sequencing on compatible platforms.

Note: KAPA Dual-Indexed Adapters are not methylated, and can therefore not be used for methyl-seq applications.

Procedure for handling Dual-Indexed Adapter Plate

• Remove the KAPA Dual-Indexed Adapter plate from its packaging sleeve and thaw at room temperature for 10 min.
• Centrifuge the KAPA Dual-Indexed Adapter plate at 280 x g for 1 min at room temperature.
• Carefully remove the foil cover to avoid cross- contamination of the dual-indexed adapters. Discard the foil cover.
• Remove the desired volume of each dual-indexed adapter by following the guidelines in Important Parameters (pp. 3 – 7).
• If you are not processing the entire KAPA Dual-Indexed Adapter plate at the same time, apply a new adhesive foil seal (3 are supplied in the KAPA Dual-Indexed Adapter Kit).
If required, similar sealing film can be used for plates containing diluted adapters.
• Label the re-sealed Dual-Indexed Adapter plate and store at -15 °C to -25 °C in a constant-temperature freezer.

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