适用说明:本说明书适用于KAPA Library Preparation Kits for Ion Torrent (07961952001 与 07961979001), 和 KAPA Library Preparation Kits for Ion Torrent for PCR-free workflows (07961987001 与 07961995001). 友情提示: 1)本文档中的页码,附录均指原说明书中的页码及附录; 2)本文档包含原说明书中的产品描述,产品应用及实验操作流程、故障排查等主要信息,欲了解关于本产品详细说明及信息,建议参考原说明书 (点击面板上“下载PDF”可下载)。
Product Description
This KAPA Library Preparation Kit is designed for the preparation of libraries for sequencing on the Ion Personal Genome Machine (PGM) and Ion Proton semiconductor sequencers. The kit provides all of the enzymes and reaction buffers required for constructing libraries from 100 ng – 1 µg of fragmented, double-stranded DNA via the following steps:
The kit provides all of the enzymes and buffers required for library construction and amplification, but does not include adapters or beads. Enzyme formulations and reaction buffers for end repair and ligation and nick repair are supplied in convenient, concentrated formats. KAPA Pure Beads and Ion Torrent Adapters are available separately. These formulations ensure maximum stability of the reagents and high reaction efficiencies while simplifying reaction setup. In order to maximize sequence coverage uniformity, it is critical that library amplification bias be kept to a minimum. KAPA HiFi DNA Polymerase has been designed for low- bias, high-fidelity PCR, and is the reagent of choice for next generation sequencing (NGS) library amplification.1,2,3 The KAPA Library Preparation Kit for Ion Torrent (KK8300, KK8301) includes KAPA HiFi HotStart ReadyMix (2X) and an adapter-specific primer mix for library amplification. Kits without library amplification components (KK8310 and KK8311) are available for PCR-free workflows.
Product Applications
The KAPA Library Preparation Kit for Ion Torrent Platforms is ideally suited for NGS library construction workflows that involve end repair, adapter ligation and nick repair, and library amplification (optional). The protocol may be adapted for incorporation into workflows for a wide range of NGS applications, including: ● targeted sequencing by solution hybrid selection, ● amplicon sequencing, ● whole-genome shotgun sequencing, ● RNA-seq, and ● ChIP-seq.
Library Preparation Protocol
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