摘要:Small RNA是生物体内一类重要的功能分子,包括miRNA、siRNA和piRNA。它的主要功能是诱导基因沉默,调控细胞生长、发育、基因转录和翻译等生物学过程。基于新一代高通量测序技术的small RNA测序,可一次性获得数百万条small RNA序列,能够快速地鉴定某种组织在特定状态下的所有已知small RNA,并发现新的small RNA,为small RNA功能研究提供有力工具。本实验教程以miRNA为例,详述了small RNA测序数据的分析流程。
关键词: miRNA-seq, 测序定量, 差异表达, 靶标预测
仪器设备
软件
实验步骤
注:
参考文献
If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.