实验原理及目的:植物涂片法指将植物材料酶解去掉细胞壁后,经低渗液处理,用针尖敲碎后在玻片上平铺展开,火焰干燥使染色体膨胀,得到分散良好、背景干净的染色体和细胞核,又称低渗火焰干燥法。涂片制片法在植物细胞生物学、细胞遗传学、分子细胞生物学等研究中应用广泛,主要进行染色体计数、核型分析、荧光原位杂交等相关实验。
关键词: 涂片, 染色体, 细胞学
材料与试剂
仪器设备
实验步骤
溶液配方
参考文献
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