实验原理:黄酮是一类常见的次生代谢产物,它们广泛地存在于自然界中某些植物体内,并且具有多种生物活性,最主要包括抗氧化、抗病菌等。高效液相色谱有对代谢物进行分离的功能,质谱可以对代谢物进行鉴定,对于水稻叶片中大量的黄酮类物质,可以利用高效液相色谱-质谱联用技术进行检测和鉴定。 实验目的:通过液相色谱-质谱联用技术对水稻叶片中常见的15种黄酮进行检测和鉴定。
关键词: 水稻, 代谢物, 液相色谱-质谱联用
材料与试剂
仪器设备
实验步骤
结果与分析
注意事项
参考文献
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