Microbiology

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    Heterologous Expression and Purification of SARS-CoV2 Nucleocapsid Protein
    Authors:  Ankur Garg, Lihong Liu, David D. Ho and Leemor Joshua-Tor, date: 08/05/2020, view: 952, Q&A: 0
    This protocol describes a step by step method for heterologous expression of SARS-CoV2 Nucleocapsid (N) protein in Escherichia coli. Moreover, this protocol includes steps to purify the N protein to high purity and homogeneity. Thus, ...
    Different Methods of Soil DNA Extraction
    Authors:  Asmita Kamble and Harinder Singh, date: 01/20/2020, view: 2195, Q&A: 0
    Soil is the major reservoir of microbial diversity. Only 1% of microbial diversity can be cultured while 99% is still not culturable. It is necessary to extract DNA from soil in order to explore the 99% microbial diversity, which will be useful to ...
    One Plate-double Nutrient Endospore Activation Method
    Authors:  Bhagwan Narayan Rekadwad, Juan M. Gonzalez and Chandrahasya N. Khobragade, date: 01/05/2020, view: 868, Q&A: 0
    This paper presents a simple, swift, improved and inexpensive one plate-double nutrient endospore activation method (ODNEAM) for activation of thermophilic endospore (thermospore). Revival and cultivation of dormant bacteria experiencing ...
    Microfluidic-based Growth and Imaging of Bacterial Biofilms
    Authors:  James M Brown, Birte Blunk, Paul Williams and Kim R Hardie, date: 12/20/2019, view: 1138, Q&A: 0
    Biofilms are a ubiquitous form of growth for bacteria on surfaces in most environments, natural or manmade. Here we present a protocol using the Bioflux microfluidic system to investigate the organized structure and development of these ...
    Isolation of PCR-quality Genomic DNA from Soils Impacted with Extra Heavy Crude Oil
    For the study of microbial communities in samples of soils impacted with extra heavy crude oil, it is necessary to perform molecular analyses. Due to the difficulty of oil matrix handling, there are very few protocols reported in writing. Also, one ...
    Very Low Molecular Weight Proteins Electrophoresis Protocol
    Authors:  César E. Rivera, José D. Rosales, Juan C. Freites-Perez and Eliaira Rodriguez, date: 11/20/2018, view: 6605, Q&A: 3
    The electrophoresis is the most used technique to separate proteins and usually the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) proposed by Laemmli is the prefered since it can diferentiate large size proteins, but for very ...
    Ty1 Retrotransposition Frequency Assay Using a Chromosomal Ty1his3AI or Ty1kanMXAI Element
    Author:  M. Joan Curcio, date: 09/05/2018, view: 2514, Q&A: 0
    Here I describe a simple genetic assay to determine the frequency of retrotransposition of a single chromosomal Ty1 element that is marked with the retrotransposition indicator gene, his3AI or kanMXAI. The assay is used to ...
    Assays for Oxidative Responses of Fusarium graminearum Strains to Superoxide Radicals
    Authors:  Yan-Zhang Wang, Yan Guo and Wei-Hua Tang, date: 09/05/2018, view: 2627, Q&A: 0
    The ascomycete fungus Fusarium graminearum is a major causal agent of Fusarium head blight (FHB), a devastating disease affecting small grains cereals worldwide. To better understand the pathogenesis of this fungus, we provide here ...
    Growth of Chlamydomonas reinhardtii under Circadian Conditions
    Authors:  Volker Wagner and Maria Mittag, date: 08/20/2018, view: 3285, Q&A: 0
    The green biflagellate unicellular alga Chlamydomonas reinhardtii serves as a model to study fundamental biological processes such as the structure and function of flagella or light-driven processes including photosynthesis, its behavioral ...
    Generation of Fusarium graminearum Knockout Mutants by the Split-marker Recombination Approach
    Authors:  Wan-Qiu Wang and Wei-Hua Tang, date: 08/20/2018, view: 3177, Q&A: 0
    Fusarium graminearum is a destructive phytopathogen and shows an impressive metabolic diversity. Gene deletion is an important and useful approach for gene function study. Here we present a protocol for generating gene deletion mutant by ...



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