Microbiology

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Western Blot in Maize

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    Isolation of PCR-quality Genomic DNA from Soils Impacted with Extra Heavy Crude Oil
    For the study of microbial communities in samples of soils impacted with extra heavy crude oil, it is necessary to perform molecular analyses. Due to the difficulty of oil matrix handling, there are very few protocols reported in writing. Also, one ...
    Very Low Molecular Weight Proteins Electrophoresis Protocol
    Authors:  César E. Rivera, José D. Rosales, Juan C. Freites-Perez and Eliaira Rodriguez, date: 11/20/2018, view: 1802, Q&A: 1
    The electrophoresis is the most used technique to separate proteins and usually the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) proposed by Laemmli is the prefered since it can diferentiate large size proteins, but for very ...
    Ty1 Retrotransposition Frequency Assay Using a Chromosomal Ty1his3AI or Ty1kanMXAI Element
    Author:  M. Joan Curcio, date: 09/05/2018, view: 975, Q&A: 0
    Here I describe a simple genetic assay to determine the frequency of retrotransposition of a single chromosomal Ty1 element that is marked with the retrotransposition indicator gene, his3AI or kanMXAI. The assay is used to ...
    Assays for Oxidative Responses of Fusarium graminearum Strains to Superoxide Radicals
    Authors:  Yan-Zhang Wang, Yan Guo and Wei-Hua Tang, date: 09/05/2018, view: 1417, Q&A: 0
    The ascomycete fungus Fusarium graminearum is a major causal agent of Fusarium head blight (FHB), a devastating disease affecting small grains cereals worldwide. To better understand the pathogenesis of this fungus, we provide here ...
    Growth of Chlamydomonas reinhardtii under Circadian Conditions
    Authors:  Volker Wagner and Maria Mittag, date: 08/20/2018, view: 1693, Q&A: 0
    The green biflagellate unicellular alga Chlamydomonas reinhardtii serves as a model to study fundamental biological processes such as the structure and function of flagella or light-driven processes including photosynthesis, its behavioral ...
    Generation of Fusarium graminearum Knockout Mutants by the Split-marker Recombination Approach
    Authors:  Wan-Qiu Wang and Wei-Hua Tang, date: 08/20/2018, view: 1564, Q&A: 0
    Fusarium graminearum is a destructive phytopathogen and shows an impressive metabolic diversity. Gene deletion is an important and useful approach for gene function study. Here we present a protocol for generating gene deletion mutant by ...
    Fusarium graminearum Double (Triple) Mutants Generation Using Sexual Crosses
    Authors:  Yan Guo, Dong Zhang and Wei-Hua Tang, date: 08/05/2018, view: 1225, Q&A: 0
    Fusarium graminearum is a destructive phytopathogen that infects major cereal crops such as wheat, maize and barley. Double or triple mutants are often very useful in the phenotypic and genetic analysis of genes that function redundantly or ...
    Perithecium Formation and Ascospore Discharge in Fusarium graminearum
    Authors:  Yan Guo, Wan-Qian Wei, Dong Zhang and Wei-Hua Tang, date: 08/05/2018, view: 1295, Q&A: 0
    The filamentous ascomycete Fusarium graminearum (previously also known as Gibberella zeae) is a phytopathogen of grain cereals, reducing crop yield and grain quality. The abilities of sexual reproduction organ-perithecium ...
    Quantification of Root Colonizing Bacteria
    Authors:  Maged M. Saad, Axel de Zélicourt, Eleonora Rolli , Lukas Synek and Heribert Hirt , date: 07/20/2018, view: 1261, Q&A: 0
    Here we describe a simple method to quantify the number of viable bacteria (e.g., Enterobacter sp. SA187) that colonize the root system of Arabidopsis thaliana.
    Visualization of Growth and Morphology of Fungal Hyphae in planta Using WGA-AF488 and Propidium Iodide Co-staining
    Authors:  Amey Redkar, Elaine Jaeger and Gunther Doehlemann, date: 07/20/2018, view: 2315, Q&A: 0
    Fungal pathogens colonizing plants show a varying degree of symptoms. Microscopy techniques have been used to study the infection and proliferation of fungal hyphae inside the host. One of the best optimized and commonly used method is the ...