Cell Biology

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    Protocols in Current Issue
    Preparation of Doublet Microtubule Fraction for Single Particle Cryo-electron Microscopy
    Authors:  Corbin Black, Daniel Chen Dai, Katya Peri, Muneyoshi Ichikawa and Khanh Huy Bui, date: 06/05/2021, view: 161, Q&A: 0
    [Abstract]

    Over the years, studying the ultrastructure of the eukaryotic cilia/flagella using electron microscopy (EM) has contributed significantly toward our understanding of ciliary function. Major complexes in the cilia, such as inner and outer dynein arms, radial spokes, and dynein regulatory complexes, were originally discovered by EM. Classical

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    Transmission Electron Microscopy for Analysis of Mitochondria in Mouse Skeletal Muscle
    Authors:  Joseph D. McMillan and Michael A. Eisenback, date: 05/20/2018, view: 12830, Q&A: 0
    [Abstract] Skeletal muscle is the most abundant tissue in the human body and regulates a variety of functions including locomotion and whole-body metabolism. Skeletal muscle has a plethora of mitochondria, the organelles that are essential for aerobic generation of ATP which provides the chemical energy to fuel vital functions such as contraction. The number ...
    Freeze-fracture-etching Electron Microscopy for Facile Analysis of Yeast Ultrastructure
    Authors:  Takuma Tsuji and Toyoshi Fujimoto, date: 09/20/2017, view: 9915, Q&A: 0
    [Abstract] We describe a streamlined method that enables the quick observation of yeast ultrastructure by electron microscopy (EM). Yeast cells are high-pressure frozen, freeze-fractured to cut across the cytoplasm, and freeze-etched to sublimate ice in the cytosol and the organelle lumen. The cellular structures delineated by these procedures are coated by ...



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