Cell Biology

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    Overcoming Autofluorescence to Assess GFP Expression During Normal Physiology and Aging in Caenorhabditis elegans
    Authors:  Alina C. Teuscher and Collin Y. Ewald, date: 07/20/2018, view: 3282, Q&A: 0
    Green fluorescent protein (GFP) is widely used as a molecular tool to assess protein expression and localization. In C. elegans, the signal from weakly expressed GFP fusion proteins is masked by autofluorescence emitted from the intestinal ...
    Immunofluorescence Detection and F-actin Staining of MTLn3 Cells
    Author:  Huan Pang, date: 04/20/2012, view: 12031, Q&A: 0
    Epidermal growth factor (EGF)-stimulated MTLn3 cells protrusion play an important role in cell migration. Phalloidin which binds F-actin in cells is an imaging tool used with light microscopy to investigate the distribution of actin. The protocol ...
    Cell Cycle Analysis Using Propidium Iodide Staining with GFP Detection
    Author:  Hui Zhu, date: 04/05/2012, view: 18708, Q&A: 3
    Infecting mammalian cells with a GFP construct to overexpress or knockdown target genes is one of the most commonly used methods to study and manipulate gene expression. To determine the target gene function on the cell cycle, analyzing the cell ...
    Cell Cycle Analysis and GFP Expression of Zebrafish Embryos by FACS
    Author:  Lili Jing, date: 02/20/2012, view: 13520, Q&A: 0
    Cell cycle analysis in combination of GFP expression has been well used to study the cell cycle distribution of GFP labeled cells. This protocol is a method to analyze the cell cycles of GFP marked cells from zebrafish embryos.
    Flourescent Immunostaining Protocol for a-Bungorotoxin (AChRs) in Zebrafish
    Author:  Lili Jing, date: 02/20/2012, view: 10191, Q&A: 0
    Zebrafish embryo is convenient for studying neuromuscular system due to its transparency. This protocol is a rapid method to visualize the AChR clusters on the postsynaptic muscle membrane.
    Immunofluorescence Assay for S Phase Entry Using BrdU Incorporation
    Author:  Hui Zhu, date: 02/05/2012, view: 15080, Q&A: 0
    Bromodeoxyuridine (BrdU) is thymidine analogue and can incorporated into the newly synthezised DNA of S-phase cells, the antibody specific for BrdU can then be used to detect the incorporated BrdU, thus indicating cells that were actively ...
    Immunofluorescent Staining of Murine Tissue
    Author:  Zheng Liu, date: 05/05/2011, view: 13658, Q&A: 0
    Renal immune complex deposition and leukocyte infiltration are characteristic of lupus nephritis in human patients and lupus-prone mice. This protocol describes how to stain frozen sections of murine kidney to study these features using fluorescent ...