Microbiology

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Western Blot in Maize

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    Growth of Chlamydomonas reinhardtii under Circadian Conditions
    Authors:  Volker Wagner and Maria Mittag, date: 08/20/2018, view: 2132, Q&A: 0
    The green biflagellate unicellular alga Chlamydomonas reinhardtii serves as a model to study fundamental biological processes such as the structure and function of flagella or light-driven processes including photosynthesis, its behavioral ...
    A Quick and Easy Method for Making Competent Escherichia coli Cells for Transformation Using Rubidium Chloride
    Authors:  Nidhi Sharma, M. Ximena Anleu Gil and Diego Wengier, date: 11/05/2017, view: 10574, Q&A: 1
    This protocol describes a quick and efficient method to make competent E. coli cells for transformation using rubidium chloride. Commercial competent cells are expensive and this protocol provides a cheaper alternative to them.
    Purification of Adenovirus by Cesium Chloride Density Gradients
    Author:  Huan Pang, date: 04/05/2012, view: 28020, Q&A: 1
    Adenovirus are efficient gene delivery systems. The standard method for purification of adenoviral vectors is based on using a cesium chloride (CsCl) density gradient combined with ultracentrifugation. This method is suitable for ...
    The Inoue Method for Preparation and Transformation of Competent E. coli: "Ultra Competent" Cells
    Author:  Hogune Im, date: 10/20/2011, view: 29180, Q&A: 1
    This protocol differs from other procedures in that the bacterial culture is grown at 18 °C rather than the conventional 37 °C. Otherwise, the protocol is unremarkable and follows a fairly standard course. Why growing the cells at low temperature ...
    RbCl Super Competent Cells
    Author:  Xiyan Li, date: 06/05/2011, view: 19002, Q&A: 3
    This method is used to inexpensively prepare home-made competent cells of E. coli. The transformation efficiency is at the high end of chemical-efficient competent cells, and close to library-efficient competent cells.
    Yeast Vacuole Staining with FM4-64
    Author:  Zongtian Tong, date: 01/05/2011, view: 23239, Q&A: 2
    The lipophilic probe, FM 4-64 does not fluoresce much in water but fluoresces strongly after binding to the outer plasma membrane, providing clear and distinguishable plasma membrane staining. The binding is rapid and reversible. In this protocol ...