Environmental science

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    Glucose Starvation, Magnesium Ion Starvation, and Bile Stress Assays
    Authors:  Aryashree Arunima and Mrutyunjay Suar, date: 09/20/2021, view: 58, Q&A: 0

    Salmonella enterica serovar Enteritidis (S. Enteritidis) is a leading causative pathogen for food-borne gastroenteritis. During its course of infection, it confronts myriads of physiological barriers inside the host, such as nutrient deprivation,

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    Agrobacterium-mediated Transformation of Japonica Rice Using Mature Embryos and Regenerated Transgenic Plants
    Authors:  Ammar Elakhdar, Masako Fukuda and Takahiko Kubo, date: 09/20/2021, view: 42, Q&A: 0

    Identification of novel genes and their functions in rice is a critical step to improve economic traits. Agrobacterium tumefaciens-mediated transformation is a proven method in many laboratories and widely adopted for genetic engineering in rice.

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    A Standard Operative Procedure for Safe-handling of Remains and Wastes of COVID-19 Patients
    The ongoing coronavirus disease-2019 (COVID-19) pandemic has raised significant public health issues which need to be attended. To enable efficient handling of the situation and prevent the spread of the epidemic, healthcare professionals require ...
    Accelerated Snowmelt Protocol to Simulate Climate Change Induced Impacts on Snowpack Dependent Ecosystems
    Field studies that simulate the effects of climate change are important for a predictive understanding of ecosystem responses to a changing environment. Among many concerns, regional warming can result in advanced timing of spring snowmelt in ...
    Different Methods of Soil DNA Extraction
    Authors:  Asmita Kamble and Harinder Singh, date: 01/20/2020, view: 7635, Q&A: 0
    Soil is the major reservoir of microbial diversity. Only 1% of microbial diversity can be cultured while 99% is still not culturable. It is necessary to extract DNA from soil in order to explore the 99% microbial diversity, which will be useful to ...
    One Plate-double Nutrient Endospore Activation Method
    Authors:  Bhagwan Narayan Rekadwad, Juan M. Gonzalez and Chandrahasya N. Khobragade, date: 01/05/2020, view: 2078, Q&A: 0
    This paper presents a simple, swift, improved and inexpensive one plate-double nutrient endospore activation method (ODNEAM) for activation of thermophilic endospore (thermospore). Revival and cultivation of dormant bacteria experiencing ...
    Experimental Setup for a Diffusion Bioreactor to Isolate Unculturable Soil Bacteria
    Authors:  Dhiraj Kumar Chaudhary and Jaisoo Kim, date: 10/05/2019, view: 3284, Q&A: 0
    Unculturable bacteria are those bacteria which proliferate in their native habitat but unable to grow or thrive in the normal laboratory media and conditions. The molecular techniques have revealed the significance of these uncultured bacteria in ...
    Quantification of Blumenol Derivatives as Leaf Biomarkers for Plant-AMF Association
    Authors:  Elisabeth Mindt, Ming Wang, Martin Schäfer, Rayko Halitschke and Ian T. Baldwin, date: 07/20/2019, view: 3668, Q&A: 0
    Symbiotic interactions between arbuscular mycorrhizal fungi (AMF) and plants are widespread among land plants and can be beneficial for both partners. The plant is provided with mineral nutrients such as nitrogen and phosphorous, whereas it provides ...
    Detachment Procedure of Bacteria from Atmospheric Particles for Flow-cytometry Counting
    Authors:  Carolina M. Araya, Alberto Cazorla and Isabel Reche, date: 06/20/2019, view: 3193, Q&A: 0
    The protocol separates bacteria from atmospheric particles, obtaining with greater precision their abundance in the atmospheric deposition. This procedure is similar to the one used to separate bacteria in streambed sediments. The detachment ...
    Isolation of PCR-quality Genomic DNA from Soils Impacted with Extra Heavy Crude Oil
    For the study of microbial communities in samples of soils impacted with extra heavy crude oil, it is necessary to perform molecular analyses. Due to the difficulty of oil matrix handling, there are very few protocols reported in writing. Also, one ...



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