Developmental Biology

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    Seed Sterilization and Seedling Growth on Plates in the Model Grass Brachypodium distachyon
    Authors:  Aylin S Haas and Michael T. Raissig, date: 08/05/2020, view: 336, Q&A: 0
    Brachypodium distachyon is a model grass closely related to wheat and barley. New resources and methods are being developed and Brachypodium is becoming a popular model for developmental biology, cell wall biology, (phylo) ...
    Double Labeling of Microtubules and Actin Filaments in Maize Leaf Division Zone
    Authors:  Qiong Nan, Janette Mendoza and Michelle Renee Facette, date: 06/20/2019, view: 1812, Q&A: 0
    Maize leaves grow in a gradient. The base of young maize leaves contains a high proportion of symmetrically and asymmetrically dividing cells, making the leaf an effective model system for the study of cell division. Here, we describe a double ...
    Dissection and Whole Mount Staining of Retina from Neonatal Mice
    Authors:  Mitsutaka Ogawa and Tetsuya Okajima, date: 10/05/2018, view: 3801, Q&A: 0
    Here we provide a detailed protocol for whole mount staining of mouse retina. This protocol was used to analyze retinal angiogenesis in newborn mice (Sawaguchi et al., 2017) by modifying the original protocols (Powner et al., 2012; ...
    Isolation of Murine Brain and Lung Microvascular Endothelial Cells
    Authors:  Ailing Zhang, Shogo Sawaguchi, Entzu Wan, Mitsutaka Ogawa and Tetsuya Okajima, date: 08/20/2018, view: 2887, Q&A: 0
    This protocol describes how to isolate murine endothelial cells from newborn mice brain and 3-month-old mice lung by modifying the original protocols (Sobczak et al., 2010; Ruck et al., 2014). We have used the protocol to analyze ...
    Microscopic Observation of Subcellular GFP-tagged Protein Localization in Rice Anthers
    Authors:  Seijiro Ono and Ken-Ichi Nonomura, date: 08/05/2018, view: 2807, Q&A: 0
    This protocol demonstrates a simple method to determine the subcellular localization of fluorescence-tagged proteins on the vibratome sections of rice developing anthers. If a cell type-specific promoter is used to drive the tagged protein-encoding ...
    Overcoming Autofluorescence to Assess GFP Expression During Normal Physiology and Aging in Caenorhabditis elegans
    Authors:  Alina C. Teuscher and Collin Y. Ewald, date: 07/20/2018, view: 6545, Q&A: 0
    Green fluorescent protein (GFP) is widely used as a molecular tool to assess protein expression and localization. In C. elegans, the signal from weakly expressed GFP fusion proteins is masked by autofluorescence emitted from the intestinal ...
    Make Human Umbilical Vein Endothelial Cells from Cords
    Author:  Huan Pang, date: 04/20/2012, view: 11991, Q&A: 0
    Human umbilical vein endothelial cells (HUVEC) can be isolated from normal human umbilical veins, and are responsive to cytokine stimulation by expressing cell adhesion molecules. These cell systems are commonly used for physiological and ...
    Cell Culture Transfection for Production and Purification of Wnt Ligands
    Author:  Lili Jing, date: 01/20/2012, view: 18160, Q&A: 0
    Wnt ligand proteins are extremely difficult to purify and enrich in vitro. This protocol uses Wnt11r protein as an example to illustrate how to use 293T cells to produce secreted Wnt11r and collect it in vitro for further ...
    Isolation of Primary Human Keratinocytes from Foreskin
    Author:  Jason Reuter, date: 12/20/2011, view: 14240, Q&A: 0
    Keratinocytes are the primary constituents of human skin, the functional barrier between our bodies and the external environment. The balance between keratinocyte differentiation and self-renewal is crucial to skin homeostasis. Primary keratinocyte ...
    Mouse Embryonic Stem Cell Maintenance for Differentiation
    Author:  Hogune Im, date: 10/20/2011, view: 15308, Q&A: 0
    Embryonic stem cells are derived from inner cell mass of an embryo that can differentiate into every cell type in the body. Clinically, cultured red blood cell supply is of great interest. However, some of the hurdles need to be overcome. This ...



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