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Tail Tendon Break Time for the Assessment of Aging and Longitudinal Healthspan in Mice

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A Standard Operative Procedure for Safe-handling of Remains and Wastes of COVID-19 Patients

Authors: Ravi Kant Narayan, Adil Asghar, Rakesh Parashar, Chiman Kumari, Kamla Kant, Ashok Rastogi and Ashutosh Kumar, date: 10/20/2020, view: 424, Q&A: 0

The ongoing coronavirus disease-2019 (COVID-19) pandemic has raised significant public health issues which need to be attended. To enable efficient handling of the situation and prevent the spread of the epidemic, healthcare professionals require ...

Immunophenotyping and Intracellular Staining of Fixed Whole Blood for Mass Cytometry (CyTOF)

Authors: Sangeeta Kowli and Holden Maecker, date: 09/05/2020, view: 1542, Q&A: 0

In this report, we present the implementation of mass cytometry for intracellular staining using fixed whole blood. In our assay described here, 250 µl of whole blood, is stimulated in vitro with PMA/ionomycin (or left unstimulated), in the ...

Protein- and Sequencing-based Massively Parallel Single-cell Approaches to Gene Expression Profiling

Authors: Xuhuai Ji, Igor Goncharov, Miao Zhao, Molly Miranda and Holden T. Maecker, date: 02/05/2019, view: 4709, Q&A: 0

Understanding cellular diversity and dynamics in immune cells are critical for elucidating mechanisms of diagnosis, response to therapeutics, and patient prognosis. Our goal is to apply a technically simple approach for gene expression cytometry ...

Immunohistochemistry Staining for Tumor-associated Macrophage Polarization in Murine Subcutaneous Colon Tumor Allografts

Authors: Gage J. Greening, Shelby N. Bess and Timothy J. Muldoon, date: 12/05/2018, view: 6277, Q&A: 0

Tumor-associated macrophages are the most abundant immune cells in the tumor microenvironment and may express both anti-tumor and pro-tumor functions depending on their polarization towards an M1 (classical) or M2 (alternative) phenotype, ...

A Lentiviral Pseudotype ELLA for the Measurement of Antibodies Against Influenza Neuraminidase

Authors: Fabrizio Biuso, George Carnell, Emanuele Montomoli and Nigel Temperton, date: 07/20/2018, view: 3854, Q&A: 0

This protocol describes the rapid and safe production of lentiviral pseudotypes characterized by a lentiviral core containing a reporter, in conjunction with avian influenza haemagglutinin (HA) and human neuraminidase (NA) glycoproteins on the ...

Cryopreservation of Human Serum

Authors: Yael Rosenberg-Hasson and Holden Maecker, date: 11/05/2015, view: 7011, Q&A: 0

This protocol describes how to collect and aliquot human serum and store at -80 °C for future cytokine/chemokine/protein profile analysis.
Please use personal protective equipment (PPE) as required when handling samples with potential ...

Site Density Protocol

Author: Jun Huang, date: 07/05/2012, view: 7330, Q&A: 0

The site densities of cell surface molecules are useful information for cell function analysis. Using antibody staining and commercial available calibration beads, this assay quantitatively determines the T cell receptor site density at the single T ...

Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-histone Antibodies

Author: Zheng Liu, date: 06/20/2012, view: 10469, Q&A: 0

Autoimmune disease is characterized with the break of tolerance against self antigens. Anti-histone antibodies can be found in the majority of patients with system lupus erythematosus (SLE) and a number of lupus-prone mouse strains. This protocol ...

ELISA Measurement of Mouse IL-2

Author: Guo N. Huang, date: 01/05/2012, view: 10213, Q&A: 0

Interleukin-2 (IL-2) is a cytokine secreted by T cells that is essential for immune system activation. This protocol is routinely used for quantification of IL-2 concentration in the supernant of cultured lymphocytes under various stimulations and ...

Immunofluorescence on Frozen Tissue Sections

Author: Jason Reuter, date: 12/20/2011, view: 28170, Q&A: 0

Immunofluorescence is commonly used to determine the cellular or tissue localization of a protein of interest. Immunofluorescence can also be used as a qualitative measure of protein expression.

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