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    Protein- and Sequencing-based Massively Parallel Single-cell Approaches to Gene Expression Profiling
    Authors:  Xuhuai Ji, Igor Goncharov, Miao Zhao, Molly Miranda and Holden T. Maecker, date: 02/05/2019, view: 2835, Q&A: 0
    Understanding cellular diversity and dynamics in immune cells are critical for elucidating mechanisms of diagnosis, response to therapeutics, and patient prognosis. Our goal is to apply a technically simple approach for gene expression cytometry ...
    Immunohistochemistry Staining for Tumor-associated Macrophage Polarization in Murine Subcutaneous Colon Tumor Allografts
    Authors:  Gage J. Greening, Shelby N. Bess and Timothy J. Muldoon, date: 12/05/2018, view: 3565, Q&A: 0
    Tumor-associated macrophages are the most abundant immune cells in the tumor microenvironment and may express both anti-tumor and pro-tumor functions depending on their polarization towards an M1 (classical) or M2 (alternative) phenotype, ...
    A Lentiviral Pseudotype ELLA for the Measurement of Antibodies Against Influenza Neuraminidase
    Authors:  Fabrizio Biuso, George Carnell, Emanuele Montomoli and Nigel Temperton, date: 07/20/2018, view: 2683, Q&A: 0
    This protocol describes the rapid and safe production of lentiviral pseudotypes characterized by a lentiviral core containing a reporter, in conjunction with avian influenza haemagglutinin (HA) and human neuraminidase (NA) glycoproteins on the ...
    Cryopreservation of Human Serum
    Authors:  Yael Rosenberg-Hasson and Holden Maecker, date: 11/05/2015, view: 5807, Q&A: 0
    This protocol describes how to collect and aliquot human serum and store at -80 °C for future cytokine/chemokine/protein profile analysis.
    Please use personal protective equipment (PPE) as required when handling samples with potential ...
    Site Density Protocol
    Author:  Jun Huang, date: 07/05/2012, view: 6553, Q&A: 0
    The site densities of cell surface molecules are useful information for cell function analysis. Using antibody staining and commercial available calibration beads, this assay quantitatively determines the T cell receptor site density at the single T ...
    Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-histone Antibodies
    Author:  Zheng Liu, date: 06/20/2012, view: 9500, Q&A: 0
    Autoimmune disease is characterized with the break of tolerance against self antigens. Anti-histone antibodies can be found in the majority of patients with system lupus erythematosus (SLE) and a number of lupus-prone mouse strains. This protocol ...
    ELISA Measurement of Mouse IL-2
    Author:  Guo N. Huang, date: 01/05/2012, view: 9197, Q&A: 0
    Interleukin-2 (IL-2) is a cytokine secreted by T cells that is essential for immune system activation. This protocol is routinely used for quantification of IL-2 concentration in the supernant of cultured lymphocytes under various stimulations and ...
    Immunofluorescence on Frozen Tissue Sections
    Author:  Jason Reuter, date: 12/20/2011, view: 24839, Q&A: 0
    Immunofluorescence is commonly used to determine the cellular or tissue localization of a protein of interest. Immunofluorescence can also be used as a qualitative measure of protein expression.
    In vitro Differentiation of Mouse Th0, Th1 and Th2 from Naïve CD4 T Cells
    Author:  Jia Li, date: 11/20/2011, view: 48463, Q&A: 20
    In vitro differentiation of helper T cells of various lineages is frequently used in T helper cell study. Naïve CD4 T cells can differentiate into certain lineage of T help cells in vitro in the presence of specific stimulatory ...
    Naïve T Lymphocyte Infection by Murine Stem Cell Virus (MSCV) Protocol
    Author:  Jia Li, date: 11/20/2011, view: 10588, Q&A: 0
    This assay can be used for studying genes related to lymphocyte proliferation and differentiation in vitro. Stimulating lymphocytes to proliferate is important for the infection efficiency of MSCV. After overnight culture, if naïve cells ...




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