Microbiology

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    Protocols in Current Issue
    Tracking Root Interactions System (TRIS) Experiment and Quality Control
    Authors:  Hassan Massalha, Elisa Korenblum, Orr H. Shapiro and Asaph Aharoni, date: 04/20/2019, view: 1619, Q&A: 0
    [Abstract] Soil organisms are diverse taxonomically and functionally. This ecosystem experiences highly complex networks of interactions, but may also present functionally independent entities. Plant roots, a metabolically active hotspot in the soil, take an essential part in shaping the rhizosphere. Tracking the dynamics of root-microbe interactions at high ...
    Quantification of the Composition Dynamics of a Maize Root-associated Simplified Bacterial Community and Evaluation of Its Biological Control Effect
    Authors:  Ben Niu and Roberto Kolter, date: 06/20/2018, view: 3020, Q&A: 0
    [Abstract] Besides analyzing the composition and dynamics of microbial communities, plant microbiome research aims to understanding the mechanism of plant microbiota assembly and their biological functions. Here, we describe procedures to investigate the role of bacterial interspecies interactions in root microbiome assembly and the beneficial effects of the ...
    Capturing Z-stacked Confocal Images of Living Bacteria Entering Hydathode Pores of Cauliflower
    Authors:  Aude Cerutti and Alain Jauneau, date: 10/20/2017, view: 4458, Q&A: 0
    [Abstract] The present protocol to visualize living bacteria at the pore level of cauliflower hydathodes is simple and trained users in confocal microscopy can execute it successfully. It can be easily adapted to capture images with other plant-microorganism interactions at the leaf surface and should be useful to obtain important information on pore and ...
    Inoculation of Rice with Different Pathogens: Sheath Blight (Rhizoctonia solani), Damping off Disease (Pythium graminicola) and Barley Powdery Mildew (Blumeria graminis f. sp. hordei)
    Authors:  Rhoda Delventhal, Marco Loehrer, Denise Weidenbach and Ulrich Schaffrath, date: 12/20/2016, view: 7372, Q&A: 0
    [Abstract] To prevent yield losses in plant cultivation due to plant pathogens, it is an important task to find new disease resistance mechanisms. Recently, Weidenbach et al. (2016) reported about the capacity of the rice gene OsJAC1 to enhance resistance in rice and barley against a broad spectrum of different pathogens. Here, we describe ...
    Bacterial Growth Inhibition Assay for Xanthomonas oryzae pv. oryzae or Escherichia coli K12 Grown together with Plant Leaf Extracts
    Authors:  Marco Loehrer, Rhoda Delventhal, Denise Weidenbach and Ulrich Schaffrath, date: 12/20/2016, view: 4996, Q&A: 0
    [Abstract] We performed a growth inhibition assay to test antibacterial compounds in leaf extracts from transgenic rice plants. The assay is based on over-night co-incubation of a defined concentration of colony forming units (cfu) of the respective bacteria together with aqueous extracts of ground leaf tissue.
    Assessment of Wheat Resistance to Fusarium graminearum by Automated Image Analysis of Detached Leaves Assay
    Authors:  Alexandre Perochon and Fiona M. Doohan, date: 12/20/2016, view: 5808, Q&A: 0
    [Abstract] Fusarium head blight (FHB) caused by Fusarium pathogens is a globally important cereal disease. To study Fusarium pathogenicity and host disease resistance, robust methods for disease assessment and quantification are needed. Here we describe the procedure of a detached leaves assay emphasizing the image analysis. The ...
    An Assay to Study Botrytis cinerea-infected Grapevine Leaves Primed with Pseudomonas fluorescens
    [Abstract] Grapevine (Vitis vinifera L.) is susceptible to an array of diseases among them the grey mold caused by the necrotrophic fungus Botrytis cinerea that decreases grape productivity and quality. To ensure a satisfactory yield and harvest quality numerous chemical fungicides are required, but they have serious drawbacks. One ...
    Ustilago maydis Virulence Assays in Maize
    Authors:  Amey Redkar and Gunther Doehlemann, date: 03/20/2016, view: 6629, Q&A: 0
    [Abstract] The basidiomycetous smut fungus Ustilago maydis (U. maydis) infects all aerial parts of its host plant maize (Zea mays L.). Infection symptoms are seen in the form of prominent tumors on all aerial parts of maize, after the establishment of a biotrophic interaction with the host usually around 5-6 days post infection ...
    EdU Based DNA Synthesis and Cell Proliferation Assay in Maize Infected by the Smut Fungus Ustilago maydis
    Authors:  Amey Redkar and Gunther Doehlemann, date: 03/20/2016, view: 5173, Q&A: 0
    [Abstract] The basidiomycetous smut fungus Ustilago maydis (U. maydis) infects all aerial parts of its host plant maize (Zea mays L.). Infection is seen in the form of prominent tumorous symptoms after the establishment of a biotrophic interaction with the host, usually around 5-6 days after infection. The fungus colonizes the ...
    A Reliable Method for Phytophthora cajani Isolation, Sporangia, Zoospore Production and in Planta Infection of Pigeonpea
    Authors:  Mamta Sharma and Raju Ghosh, date: 01/20/2016, view: 7325, Q&A: 0
    [Abstract] Pigeonpea (Cajanus cajan L.) is an important legume crop of rainfed agriculture. High levels of protein in pigeonpea make it a valuable protein source for developing countries. Phytophthora blight caused by Phytophthora cajani (P. cajani) is a potential threat to pigeonpea (Cajanus cajan L.) production, ...



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