Chase Assay of Protein Stability in Haloferax volcanii
Authors: Xian Fu and
Julie A. Maupin-Furlow,
date: 03/20/2017,
view: 3505,
Q&A: 0
[Abstract] Highly regulated and targeted protein degradation plays a fundamental role in almost all cellular processes. Determination of the protein half-life by the chase assay serves as a powerful and popular strategy to compare the protein stability and study proteolysis pathways in cells. Here, we describe a chase assay in Haloferax volcanii, a ...
Preparation of Parasite Protein Extracts and Western Blot Analysis
Authors: Arlett Heiber and
Tobias Spielmann,
date: 06/05/2014,
view: 15968,
Q&A: 0
[Abstract] In order to prepare protein extracts of Plasmodium falciparum blood stages for western blot analysis, infected red blood cells (iRBC) need to be separated from uninfected red blood cells (uRBC) which make up the bulk of the parasite culture. Depending on the localisation of the parasite protein of interest, different methods are available ...
Western Blotting for Staphylococcus aureus AgrA
Authors: Chikara Kaito and
Kazuhisa Sekimizu,
date: 03/20/2014,
view: 8278,
Q&A: 0
[Abstract] Staphylococcus aureus has a quorum sensing system to regulate the expression of various virulence factors, which is exerted by the agr locus that encodes agrBDCA and a regulatory RNA called RNAIII. AgrB, AgrD, and AgrC proteins are involved in producing and recognizing extracellular quorum sensing molecules and transduce ...
Co-immunoprecipitation of Flag-TLR3 or Myc-MSR1 with HCV RNA
[Abstract] Co-immunoprecipitation assay of TLR3-Flag or Myc-MSR1 with HCV RNA is used to identify direct interaction of viral RNA with host proteins that recognize viral RNA to initiate interferon (IFN) signaling, a crucial antiviral response of the host cells. Both Toll-like receptor 3 (TLR3) and class-A scavenger receptor type 1 (MSR1) proteins recognize ...
Shigella IpaD and IpaB Surface Localizations
[Abstract] Shigella uses a type III secretion system to invade host cell and to cause disease. Secretion control and insertion of a translocation pore into cell membrane are critical steps for pathogenesis and are tightly linked to the formation of the needle tip complex formed by the IpaB and IpaD proteins (Veenendaal et al., 2007). ...
Virus Overlay Assay (Far-Western blotting)
[Abstract] Virus overlay assay is a method to detect protein-protein interaction in vitro. We performed the virus overlay assay to identify the receptor proteins interacting with the infectious spleen and kidney necrosis virus (ISKNV).