Modesto Redrejo-Rodriguez Biochemistry Department, Madrid Autonomous University

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Margarita Salas Centro de Biología Molecular Severo Ochoa, Centro de Biología Molecular Severo Ochoa, Spain, Spain,
1 protocol

Mónica Berjón-Otero Present address: Max Planck Institute for Medical Research, Germany, Germany,
1 protocol

Ana Lechuga Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones científicas y Universidad Autónoma de Madrid, Spain, Spain,
1 protocol

María Salas Centro de Biología Molecular Severo Ochoa, CSIC/UAM, Spain
1 protocol

Adison Wong Singapore Institute of Technology
1 protocol

Alba Blesa Universidad Francisco de Vitoria
41 protocols

Anna Köhler Georg-August-Universität Göttingen
2 protocols

Arsalan Daudi University of California
157 protocols

Editor

Modesto Redrejo-Rodriguez

Assistant Professor, Biochemistry Department, Madrid Autonomous University

Research focus

Molecular biology
DNA Replication and Repair mechanisms of viruses and mobile elements

2 Author merit
Reviewer merit
Editor merit

Education

PhD, Universidad Autónoma de Madrid (Spain), 2009

Lab information

R'n'R lab
https://mredrejo.wixsite.com/rnrlab

Publications

https://www.ncbi.nlm.nih.gov/sites/myncbi/modesto.redrejo%20rodriguez.1/bibliography/41957182/public/?sort=date&direction=descending

2 Protocols published

Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay

Authors: Ana Lechuga, Mónica Berjón-Otero, Margarita Salas and Modesto Redrejo-Rodríguez, date: 01/05/2018, view: 8586, Q&A: 0

This protocol analyzes the direct interaction between two DNA-binding proteins by pull-down co-immunoprecipitation. One of the proteins is overexpressed in E. coli as HA-tagged recombinant protein and cell-free extracts are ...

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Determination of Mutation Frequency During Viral DNA Replication

Authors: Modesto Redrejo-Rodríguez, Javier M. Rodríguez, Cristina Suárez and María L. Salas, date: 03/20/2014, view: 8333, Q&A: 0

This protocol is a simple method for evaluating mutation frequency during African swine fever virus (ASFV) replication, although it could be used also for other DNA viruses (poxvirus, herpesvirus, mimivirus, etc) with minor modifications. ...

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10 Protocols reviewed

Giant Mimiviridae CsCl Purification Protocol

Authors: Lionel Bertaux, Audrey Lartigue and Sandra Jeudy, date: 11/20/2020, view: 1878, Q&A: 0

While different giant viruses’ purification protocols are available, they are not fully described and they use sucrose gradient that does not reach an equilibrium. Here, we report a protocol for the purification of members of the Mimiviridae ...

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Quantification of Densities of Bacterial Endosymbionts of Insects by Real-time PCR

Author: Daisuke Kageyama, date: 10/05/2017, view: 8695, Q&A: 0

Increased attention has been paid to the endosymbiotic bacteria of insects. Because most insect endosymbionts are uncultivable, quantitative PCR (qPCR) is a practical and convenient method to quantify endosymbiont titers. Here we report a protocol ...

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20 Protocols edited

Protein- and Sequencing-based Massively Parallel Single-cell Approaches to Gene Expression Profiling

Authors: Xuhuai Ji, Igor Goncharov, Miao Zhao, Molly Miranda and Holden T. Maecker, date: 02/05/2019, view: 7185, Q&A: 0

Understanding cellular diversity and dynamics in immune cells are critical for elucidating mechanisms of diagnosis, response to therapeutics, and patient prognosis. Our goal is to apply a technically simple approach for gene expression cytometry ...

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Quantitative Transformation Efficiency Assay for Bacillus subtilis

Authors: Christian L. Loyo and Briana M. Burton, date: 12/05/2018, view: 6592, Q&A: 0

Bacillus subtilis (B. subtilis) is a model Gram-positive organism used to study a variety of physiological states and stress responses, one of which is the development of competence. Competence refers to the physiological state of ...

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