Hi,

you'll find the CR from VWR at this URL:
https://uk.vwr.com/app/catalog/Product;jsessionid=6nvO4UhfRBxKo5Evg2WuAw**.node3?article_number=34140.184
I haven't found the same product from US VWR...

for CR agar plates' preparation :

- prepare a 30 g/l TSB solution with bidistilled water and dissolve 15 g/l agar.
- autoclave the medium for 15 min at 120°C (if you autoclave your medium for 20 min, sugar will caramelize and your plates will be darker, and even darkest after incubation with bacteria at 37°C)
- Add the CR at a final concentration of 250 µg/ml (stock solution is prepared in bidistilled water at a concentration of 10 mg/ml) when the bottle can be safely handled with a protective glove

I hope it'll help you.
Don't hesitate if you have more questions...

Lionel 2014-07-11 08:51:39