Developmental Biology


    Protocols in Current Issue
    In vitro Time-lapse Imaging of Primary Cilium in Migrating Neuroblasts
    [Abstract] Neuronal migration is a critical step for the development of neuronal circuits in the brain. Immature new neurons (neuroblasts) generated in the postnatal ventricular-subventricular zone (V-SVZ) show a remarkable potential to migrate for a long distance at a high speed in the postnatal mammalian brain, and are thus a powerful model to analyze the ...
    DigiTAG–a RNA Sequencing Approach to Analyze Transcriptomes of Rare Cell Populations in Drosophila melanogaster
    Authors:  Lisa Landskron, Francois Bonnay, Thomas R. Burkard and Jürgen A. Knoblich, date: 11/05/2020, view: 2249, Q&A: 0

    Cell-type specific transcriptional programs underlie the development and maintenance of organs. Not only distinct cell types within a tissue, even cells with supposedly identical cell fates show a high degree of transcriptional heterogeneity. Inevitable, low cell numbers are a major hurdle to study transcriptomes of pure cell populations. Here we

    Analysis of Enteric Neural Crest Cell Migration Using Heterotopic Grafts of Embryonic Guts
    Authors:  Rodolphe Soret and Nicolas Pilon, date: 09/05/2016, view: 6976, Q&A: 0
    [Abstract] Hirschsprung disease (HSCR), also named aganglionic megacolon, is a severe congenital malformation characterized by a lack of enteric nervous system (ENS) in the terminal regions of the bowel (Bergeron et al., 2013). As the ENS notably regulates motility in the whole gastrointestinal track, the segment without neurons remains tonically ...
    Olfactory Bulb (OB) Transplants
    Authors:  Maria Savvaki and Domna Karagogeos, date: 09/05/2016, view: 6863, Q&A: 0
    [Abstract] Transplantation in mouse brain slices is a powerful tool in order to study axon targeting and migrational events during development. Taking advantage of donors and recipients belonging to different genotypes, this technique allows researchers to assess the contribution of donor and/or recipient tissue by performing various combinations and to ...
    Differentiation of Human Embryonic Stem Cells into Cone Photoreceptors
    Authors:  Anthony Flamier, Andrea Barabino and Gilbert Bernier, date: 07/20/2016, view: 8859, Q&A: 1
    [Abstract] Photoreceptors are specialized retinal neurons able to respond to light in order to generate visual information. Among photoreceptors, cones are involved in colors discrimination and high-resolution central vision and are selectively depleted in macular degenerations and cone dystrophies. A possible therapeutic solution for these disorders is to ...
    In vitro Migration Assays for Neural Stem Cells, Intermediate Neurogenic Progenitors and Immature Neurons
    Authors:  Julia Ladewig, Philipp Koch and Oliver Brüstle, date: 01/05/2015, view: 16649, Q&A: 0
    [Abstract] In the vertebrate central nervous system (CNS), different neural precursor populations such as neural stem cells (NSCs), intermediate neurogenic progenitors (INPs) and immature neurons have to migrate from their places of birth to their location of function. Coordinated migration is mediated by direct cell-cell interactions and by extracellular ...
    Open-book Preparations from Chick Embryos and DiI Labeling of Commissural Axons
    Authors:  Nicole H. Wilson and Esther T. Stoeckli, date: 07/05/2014, view: 11681, Q&A: 0
    [Abstract] Successful neural circuit formation relies on the accurate navigation of axons towards their targets during development. Axons are guided by a combination of short-range and long-range, attractive and repulsive cues. The commissural axons of the developing spinal cord have provided an informative in vivo model for the identification of ...
    Labeling of Precursor Granule Cells in the Cerebellum by ex vivo Electroporation
    Author:  Aya Ito-Ishida, date: 06/20/2013, view: 14045, Q&A: 1
    [Abstract] This protocol will be useful to introduce the genes of interest into the cerebellar granule cells at early stages of development. Since the granule cell precursors are localized in the external granule layer before migration, DNA plasmids can be specifically incorporated into the granule cells by injecting DNA solution into the cerebellar fissures ...
    Organotypic Slice Culture of Embryonic Brain Sections
    Author:  Froylan Calderon de Anda, date: 02/05/2013, view: 15958, Q&A: 0
    [Abstract] This technique will allow using brain slices to study several aspects of cortical development (i.e. neurogenesis), as well as neuronal differentiation (i.e. neuronal migration, axon and dendrite formation) in situ. This protocol is suitable for various embryonic stages (Calderon de Anda et al., 2010; Ge et al. ...

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