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Low-viscosity Matrix Suspension Culture for Human Colorectal Epithelial Organoids and Tumoroids
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MNase Digestion for Nucleosome Mapping in Neurospora
Authors:
Cigdem Sancar
,
Gencer Sancar
and
Michael Brunner
,
date:
06/05/2016,
view:
8622,
Q&A:
0
[Abstract] Digestion of chromatin by micrococcal nuclease MNase followed by high throughput sequencing allows us to determine the location and occupancy of nucleosomes on the genome. Here in this protocol we have described optimized conditions of MNase digestion of filamentous fungus
Neurospora crassa
chromatin without a requirement of a nuclear ...
Density Gradient Centrifugation for Enrichment and Identification of GFP-tagged Chitosomal Microvesicles of Filamentous Fungi
Authors:
Jorge Verdín
,
Eddy Sánchez-León
,
Rosa Fajardo-Somera
,
Carlos A. Leal Morales
,
Salomón Bartnicki-García
and
Meritxell Riquelme
,
date:
10/05/2015,
view:
14018,
Q&A:
0
[Abstract] Density gradient centrifugation has been utilized to characterize the subcellular distribution of physiologically relevant enzymes in yeasts and filamentous fungi (Leal-Morales
et al.
, 1988; Martínez
et al.
, 1989; Kamada
et al.
, 1991). This approach is now potentiated by protein tagging and live imaging techniques, which ...
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