Brian Munsky Colorado State University Fort Collins
1 protocol

William Raymond Colorado State University Fort Collins
1 protocol

Linda Forero-Quintero Colorado State University Fort Collins
1 protocol

Tatsuya Morisaki
1 protocol

Timothy J. Stasevich
  • Faculty, Colorado State University Fort Collins
Research focus
  • Biophysics, Gene Regulation, Translation, Transcription, Fluorescence Microscopy, Single Molecule Tracking
  • 2 Author merit


PhD, University of Maryland, 2006

Lab information

Stasevich Lab


2 Protocols published
Visualization, Quantification, and Modeling of Endogenous RNA Polymerase II Phosphorylation at a Single-copy Gene in Living Cells
Authors:  Linda S. Forero-Quintero, William Raymond, Brian Munsky and Timothy J. Stasevich, date: 08/05/2022, view: 1098, Q&A: 0

In eukaryotic cells, RNA Polymerase II (RNAP2) is the enzyme in charge of transcribing mRNA from DNA. RNAP2 possesses an extended carboxy-terminal domain (CTD) that gets dynamically phosphorylated as RNAP2 progresses through the transcription cycle,

A Multi-color Bicistronic Biosensor to Compare the Translation Dynamics of Different Open Reading Frames at Single-molecule Resolution in Live Cells
Authors:  Amanda L. Koch, Tatsuya Morisaki and Timothy J. Stasevich, date: 07/20/2021, view: 2260, Q&A: 0

Here, we describe how to image and quantitate the translation dynamics of a bicistronic biosensor that we recently created to fairly compare cap-dependent and IRES-mediated translation at single-molecule resolution in live human cells. This

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