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A high-quality database for basic life science protocols
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A high-quality database for basic life science protocols
Cell Biology
Latest Protocol Video
Tail Tendon Break Time for the Assessment of Aging and Longitudinal Healthspan in Mice
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Cellular Retention Assay to Determine the Interaction Frequency of CD19-directed Chimeric Antigen Receptor (CAR) Engineered Cells against CD19
+
Leukemic Cells
Authors:
Marvin I. De los Santos
and
Samuel D. Bernal
,
date:
09/05/2019,
view:
2170,
Q&A:
0
Cancer recognition by chimeric antigen receptor T (CAR-T) cell is prerequisite for cancer killing to occur. Anomalies in the binding specificity of scFv in the CAR construct may prevent successful eradication of these cancer cells. In some cases, ...
Protein- and Sequencing-based Massively Parallel Single-cell Approaches to Gene Expression Profiling
Authors:
Xuhuai Ji
,
Igor Goncharov
,
Miao Zhao
,
Molly Miranda
and
Holden T. Maecker
,
date:
02/05/2019,
view:
5249,
Q&A:
0
Understanding cellular diversity and dynamics in immune cells are critical for elucidating mechanisms of diagnosis, response to therapeutics, and patient prognosis. Our goal is to apply a technically simple approach for gene expression cytometry ...
Ty1 Retrotransposition Frequency Assay Using a Chromosomal
Ty1his3AI
or
Ty1kanMXAI
Element
Author:
M. Joan Curcio
,
date:
09/05/2018,
view:
3245,
Q&A:
0
Here I describe a simple genetic assay to determine the frequency of retrotransposition of a single chromosomal Ty1 element that is marked with the retrotransposition indicator gene,
his3AI
or
kanMXAI
. The assay is used to ...
High-throughput YO-PRO-1 Uptake Assay for P2X7 Receptors Expressed in HEK Cells
Authors:
Akira Karasawa
and
Toshimitsu Kawate
,
date:
07/20/2018,
view:
4806,
Q&A:
0
P2X7 receptors are extracellular ATP-gated ion channels that play broad physiological and pathological roles in animals (Sluyter, 2017). Activation of P2X7 receptors lead to the opening of membrane channels permeable for small cations like Na
+
...
Quantifying Gene Expression Directly from FACS Using Hydrolysis (TaqMan) Probes on Flash-frozen Cells
Authors:
Kirsten A. Copren
,
Oyang Teng
,
Cassandra J. Adams
,
Cláudia Bispo
and
Michael Lee
,
date:
04/20/2018,
view:
5942,
Q&A:
0
The TaqMan Gene Expression Cells-to-Ct
TM
Kit enables reverse transcription directly from cell lysates without the need for isolating RNA. The recommended input range for the lysis reaction is between 10-10
5
cells, though the ...
Cell Proliferation Assay by Flow Cytometry (BrdU and PI Staining)
Author:
Hui Zhu
,
date:
04/05/2012,
view:
63539,
Q&A:
2
Cell Proliferation assays include an important set of fluorescence-based tests that can monitor cell health and cell division by evaluating DNA synthesis through thymidine incorporation. Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a ...
Calcium Imaging
Author:
Mingye Feng
,
date:
04/05/2012,
view:
33729,
Q&A:
5
This is a protocol of Ca
2+
imaging experiment using Ca
2+
indicator Fura-2. Ca
2+
imaging is an efficient and quantitative method for measuring cytosolic and internal store Ca
2+
levels, as well as their ...
Protocol to Determine Mitotic Index by FACS
Author:
Hui Zhu
,
date:
03/20/2012,
view:
17411,
Q&A:
0
Fluorescence Activated Cell Sorting (FACS) is a sensitive method to count mitotic cells. Cells are stained with an antibody that recognizes an antigen present only in mitotic cells, combined with propidium iodide (PI) to stain DNA. Two-dimensional ...
Cell Cycle Analysis and GFP Expression of Zebrafish Embryos by FACS
Author:
Lili Jing
,
date:
02/20/2012,
view:
15880,
Q&A:
0
Cell cycle analysis in combination of GFP expression has been well used to study the cell cycle distribution of GFP labeled cells. This protocol is a method to analyze the cell cycles of GFP marked cells from zebrafish embryos.
Sucrose Gradient Analysis of Proteins
Author:
Hui Zhu
,
date:
02/05/2012,
view:
27585,
Q&A:
1
The sedimentation rate of a protein in a linear sucrose gradient can be used to determine its S value when compared to proteins of known S values which are run in a parallel gradient. This can be used to estimate an approximate molecular weight of ...
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