Plant Science

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    A Low-volume Hydroponic Protocol for Maize and A Sensitive Bud-length Assay for Root-to-Shoot Impacts of The Strigolactone Analog, rac-GR24
    Authors:  Jiahn-Chou Guan and Karen E. Koch, date: 08/20/2020, view: 266, Q&A: 0
    Hydroponic approaches have been a boon for root research by facilitating root-feeding studies as well as secretion analysis. Results have aided our understanding of root-shoot signaling, transport, hormone function and more. However, existing ...
    Seed Sterilization and Seedling Growth on Plates in the Model Grass Brachypodium distachyon
    Authors:  Aylin S Haas and Michael T. Raissig, date: 08/05/2020, view: 459, Q&A: 0
    Brachypodium distachyon is a model grass closely related to wheat and barley. New resources and methods are being developed and Brachypodium is becoming a popular model for developmental biology, cell wall biology, (phylo) ...
    Toluidine Blue O Staining of Paraffin-Sectioned Maize Tissue
    Authors:  Josh Strable, Jeffrey R. Yen, Michael J. Scanlon and Anne W. Sylvester, date: 05/05/2020, view: 774, Q&A: 0
    Histology incorporates microtechnique, the preparation of living material for observation and microscopy to study the anatomy of cells, tissues and organs. Interpreting histological patterns in a specimen may help to correlate form and function at ...
    In vitro Conditions for Dark Growth and Analysis of Maize Seedlings
    Authors:  Jeffrey Farrow, Marschal A. Bellinger and Carolyn G. Rasmussen, date: 03/20/2020, view: 786, Q&A: 0
    Maize is an important model organism for understanding plant traits essential for proper growth and germination. One type of growth, skotomorphogenesis, occurs in the absence of light. Seedlings grown in the absence of light exhibit dramatic ...
    Staining Maize Epidermal Leaf Peels with Toluidine Blue O
    Authors:  Marschal A. Bellinger, Sukhmani K. Sidhu and Carolyn G. Rasmussen, date: 04/20/2019, view: 4099, Q&A: 0
    Maize is an important model organism for understanding cell patterning and development. The regular patterning of maize leaf epidermal cells has previously been characterized using the dye toluidine blue O (TBO). The polychromatic dye TBO ...
    Glue Impressions of Maize Leaves and Their Use in Classifying Mutants
    Authors:  Lindy A. Allsman, Raquel N. Dieffenbacher and Carolyn G. Rasmussen, date: 04/05/2019, view: 2337, Q&A: 0
    Maize cells are surrounded by cell walls that can be easily visualized using a simple protocol outlined below. A glue impression is an imprint of maize epidermal cells on a glass slide made using a cyanoacrylate-based glue (super glue) that produces ...
    Arabidopsis Growing Protocol – A General Guide
    Author:  Xiyan Li, date: 09/05/2011, view: 31750, Q&A: 1
    Arabidopsis as the model organism for higher plants is widely studied among plant biology labs around the world. However, taking care of this tiny plant may not be trivial. Here is a general guide used for the Heven Sze lab at the University of ...
    Arabidopsis Stomatal Opening and Closing Experiment
    Author:  Zhixin Zhao, date: 05/20/2011, view: 16261, Q&A: 1
    Stomata pores are mainly localized in the lower epidermis of plant leaves and transpirational water loss occurs through these pores. ABA inhibits light-promoted stomatal opening and promotes stomatal closure. This protocol describes how to measure ...



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