Plant Science

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    Spatiotemporal Quantification of Cytosolic pH in Arabidopsis Pollen Tubes
    Authors:  Maria Teresa Portes, Daniel S.C. Damineli and José A. Feijó, date: 07/20/2021, view: 1196, Q&A: 0

    Ion-specific probes and fluorescent indicators have been key in establishing the role of ion signaling, namely calcium, protons, and anions, in plant development, providing a robust approach for monitoring spatiotemporal changes in intracellular ion

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    Rice Root Hair Phenotypes Imaged by Cryo-SEM

    Cryo-scanning electron microscopy (cryo-SEM) was first introduced for scientific use in the 1980s. Since then, cryo-SEM has become a routine technique for studying the surfaces and internal structures of biological samples with a high water content.

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    Age, Wound Size and Position of Injury – Dependent Vascular Regeneration Assay in Growing Leaves

    Recurring damage to the aerial organs of plants necessitates their prompt repair, particularly their vasculature. While vascular regeneration assays for aerial plant parts such as the stem and inflorescence stalk are well established, those for leaf

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    Histochemical Staining of Suberin in Plant Roots
    Authors:  Peter Marhavý and Shahid Siddique, date: 02/05/2021, view: 3081, Q&A: 1

    Histological stains are useful tools for characterizing cell shape, arrangement and the material they are made from. Stains can be used individually or simultaneously to mark different cell structures or polymers within the same cells, and to

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    Measuring Extracellular Proton and Anionic Fluxes in Arabidopsis Pollen Tubes
    Authors:  Maria Teresa Portes and José A. Feijó, date: 02/05/2021, view: 2483, Q&A: 0

    The ion-selective vibrating probe has been used to detect and quantify the magnitude and direction of transmembrane fluxes of several ions in a wide range of biological systems. Inherently non-invasive, vibrating probes have been essential to access

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    Development and Standardization of Rapid and Efficient Seed Germination Protocol for Cannabis sativa
    Authors:  Aleksei Sorokin, Narendra Singh Yadav, Daniel Gaudet and Igor Kovalchuk, date: 01/05/2021, view: 2564, Q&A: 0

    Cannabis seed germination is an important process for growers and researchers alike. Many biotechnological applications require a reliable sterile method for seed germination. This protocol outlines a seed germination procedure for Cannabis sativa

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    A Pulse–chase EdU Method for Detection of Cell Division Orientation in Arabidopsis and Juncus prismatocarpus Leaf Primordia
    Authors:  Xiaofeng Yin and Hirokazu Tsukaya, date: 01/05/2021, view: 1758, Q&A: 0
    In plants, the morphological diversity of leaves is largely determined by cell division, especially cell division orientation. Whereas cell division itself is easily monitored, the detection and quantification of cell division orientation are ...
    A Low-volume Hydroponic Protocol for Maize and A Sensitive Bud-length Assay for Root-to-Shoot Impacts of The Strigolactone Analog, rac-GR24
    Authors:  Jiahn-Chou Guan and Karen E. Koch, date: 08/20/2020, view: 1650, Q&A: 0
    Hydroponic approaches have been a boon for root research by facilitating root-feeding studies as well as secretion analysis. Results have aided our understanding of root-shoot signaling, transport, hormone function and more. However, existing ...
    Seed Sterilization and Seedling Growth on Plates in the Model Grass Brachypodium distachyon
    Authors:  Aylin S Haas and Michael T. Raissig, date: 08/05/2020, view: 1962, Q&A: 0
    Brachypodium distachyon is a model grass closely related to wheat and barley. New resources and methods are being developed and Brachypodium is becoming a popular model for developmental biology, cell wall biology, (phylo) ...
    Gene Expression and Genome Editing Systems by Direct Delivery of Macromolecules Into Rice Egg Cells and Zygotes
    Authors:  Erika Toda and Takashi Okamoto, date: 07/20/2020, view: 2063, Q&A: 0
    Polyethylene glycol calcium (PEG-Ca2+)-mediated transfection allows rapid and efficient examination to analyze diverse cellular functions of genes of interest. In plant cells, macromolecules, such as DNA, RNA and protein, are delivered ...



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