Hyaluronan Isolation from Mouse Mammary Gland
Authors: Cornelia Tolg,
Mary Cowman and
Eva A. Turley,
date: 06/05/2018,
view: 1359,
Q&A: 0
[Abstract] The glycosaminoglycan hyaluronan (HA) is a key component of the extracellular matrix. The molecular weight of HA is heterogeneous and can reach from several million to several hundred daltons. The effect of HA on cell behavior is size dependent; fragmented HA acts as a danger signal, stimulates cell migration and proliferation and is ...
Nucleosome Positioning Assay
Authors: Zhongliang Zhao and
Holger Bierhoff,
date: 05/20/2017,
view: 5316,
Q&A: 0
[Abstract] The basic unit of chromatin is the nucleosome, a histone octamer with 147 base pairs of DNA wrapped around it. Positions of nucleosomes relative to each other and to DNA elements have a strong impact on chromatin structure and gene activity and are tightly regulated at multiple levels, i.e., DNA sequence, transcription factor binding, ...
Liposome Flotation Assays for Phosphoinositide-protein Interaction
Authors: Helene Tronchere and
Frederic Boal,
date: 03/05/2017,
view: 8187,
Q&A: 0
[Abstract] Phosphoinositides are rare membrane lipids involved in the control of the major cellular functions and signaling pathways. They are able to recruit specific effector proteins to the cytosolic face of plasma membrane and organelles to coordinate a vast variety of signaling and trafficking processes, as well to maintain specific identity of the ...
In situ Hybridization (ISH) and Quantum Dots (QD) of miRNAs
[Abstract] miRNA are short non-coding RNA which inhibit translation of mRNA. miRNA regulate several cellular processes. Certain miRNA are known to induce oncogenesis. miRNA can be measured by real-time PCR and be imaged using a combination of in situ hybridization (ISH) and quantum dots (QD). The advantage of using quantum dots is that several miRNA ...
miRNA Characterization from the Extracellular Vesicles
[Abstract] Cancer cells and cancer associated stromal cells co-evolve secrete extracellular vesicles to the surrounding regions and regulate several processes involved in cancer metastasis. miRNAs have been known to be mediators of cancer progression and metastasis. miRNAs consist of short noncoding RNA. miRNAs are stable in extracellular fluids such as ...
Polysome Fractionation to Analyze mRNA Distribution Profiles
[Abstract] Eukaryotic cells adapt to changes in external or internal signals by precisely modulating the expression of specific gene products. The expression of protein-coding genes is controlled at the transcriptional and post-transcriptional levels. Among the latter steps, the regulation of translation is particularly important in cellular processes that ...
In vitro Assessment of RNA Polymerase I Activity
Author: Marzia Govoni,
date: 02/05/2017,
view: 3868,
Q&A: 0
[Abstract] In eukaryotic cells transcriptional processes are carried out by three different RNA polymerases: RNA polymerase I which specifically transcribes ribosomal RNA (rRNA), RNA polymerase II which transcribes protein-coding genes to yield messenger RNAs (mRNAs) and small RNAs, while RNA polymerase III transcribes the genes for transfer RNAs and for the ...