Vascular Smooth Muscle Cell Isolation and Culture from Mouse Aorta
[Abstract] Vascular smooth muscle cells (SMC) in the ascending thoracic aorta arise from neural crest cells, whereas SMCs in the descending aorta are derived from the presomitic mesoderm. SMCs play important roles in cardiovascular development and aortic aneurysm formation. This protocol describes the detailed process for explanting ascending and descending ...
Aorta Ring Assay
Authors: Jing Jin,
Xinyang Hu,
Ling Zhang and
Jian'an Wang,
date: 07/05/2016,
view: 11398,
Q&A: 1
[Abstract] Angiogenesis is the nature and pathological process of blood vessel growth from pre-existing vascular buds. It plays an important role in cancer and cardiovascular disease. The aorta ring assay is an approach to study angiogenesis. In this experiment, we used the aorta of rat as the study material, cleaned the surrounding tissue of aorta and cut ...
Mouse Retinal Whole Mounts and Quantification of Vasculature Protocol
Authors: Irit Adini and
Kaustabh Ghosh,
date: 08/05/2015,
view: 13818,
Q&A: 1
[Abstract] Angiogenesis is the formation of new blood vessels from a pre-existing vascular bed. It is a multi-step process beginning with enzymatic degradation of the capillary basement membrane, followed by endothelial cell (EC) proliferation, migration, tube formation, assembly of a new basement membrane, and pericyte stabilization. Aberrant angiogenesis ...