Cell Biology

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    Protocols in Current Issue
    Evaluation of Viable Cells in Pseudomonas aeruginosa Biofilms by Colony Count and Live/Dead Staining
    Authors:  Magdalena Pezzoni, Ramón Augusto Pizarro and Cristina Susana Costa, date: 09/20/2020, view: 238, Q&A: 0
    [Abstract] Pseudomonas aeruginosa is a human pathogen capable to form robust biofilms. P. aeruginosa biofilms represent a serious problem because of the adverse effects on human health and industry, from sanitary and economic points of view. Typical strategies to break down biofilms have been long used, such as the use of disinfectants or ...
    Multiplication and Growth Inhibition Activity Assays for the Zoonotic Malaria Parasite, Plasmodium knowlesi
    Authors:  Franziska Mohring, Thomas A. Rawlinson, Simon J. Draper and Robert W. Moon, date: 09/05/2020, view: 520, Q&A: 0
    [Abstract] Malaria remains a major cause of morbidity and mortality globally. Clinical symptoms of the disease arise from the growth and multiplication of Plasmodium parasites within the blood of the host. Thus in vitro assays to determine how drug, antibody and genetic perturbations affect the growth rate of Plasmodium parasites ...
    In vivo Tumor Growth and Spontaneous Metastasis Assays Using A549 Lung Cancer Cells
    [Abstract] Metastasis accounts for the majority of cancer related deaths. The genetically engineered mouse (GEM) models and cell line-based subcutaneous and orthotopic mouse xenografts have been developed to study the metastatic process. By using lung cancer cell line A549 as an example, we present a modified protocol to establish the cell line-based ...
    A Novel Protocol to Generate Decellularized Bovine Spinal Cord Extracellular Matrix-based Scaffolds (3D-dCBS)
    Authors:  Yavuz E. Arslan, Burcu Efe and Tugba Sezgin Arslan, date: 10/05/2019, view: 1669, Q&A: 0
    [Abstract] Extracellular matrix (ECM)-based tissue engineering scaffolds have an essential role in promoting tissue regeneration. Nerve tissue engineering aims at facilitating the repair of permanent damage to the peripheral and central nervous systems, which are difficult to heal. For this purpose, a variety of biomaterials are being developed consisting of ...
    Primary Cultures from Human GH-secreting or Clinically Non-functioning Pituitary Adenomas
    Authors:  Roberto Würth, Alessandra Pattarozzi, Federica Barbieri and Tullio Florio, date: 04/05/2018, view: 3858, Q&A: 0
    [Abstract] Pituitary adenomas are among the more frequent intracranial tumors usually treated with both surgical and pharmacological–based on somatostatin and dopamine agonists–approaches. Although mostly benign tumors, the occurrence of invasive behaviors is often detected resulting in poorer prognosis. The use of primary cultures from human pituitary ...
    Bacterial Survival in Dictyostelium
    [Abstract] We performed an assay to test the ability of different E. coli strains to survive inside amoebal cells after ingestion. In the assay we incubated bacteria together with cells of Dictyostelium discoideum for six hours. After co-incubation most of the uningested bacteria were removed by centrifugation and the remaining uningested ...
    Formaldehyde Fixation of Extracellular Matrix Protein Layers for Enhanced Primary Cell Growth
    Authors:  Natalia V. Andreeva and Alexander V. Belyavsky, date: 07/05/2017, view: 5680, Q&A: 0
    [Abstract] Coating tissue culture vessels with the components of the extracellular matrix such as fibronectin and collagens provides a more natural environment for primary cells in vitro and stimulates their proliferation. However, the effects of such protein layers are usually rather modest, which might be explained by the loss immobilized proteins ...
    Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation
    Authors:  Esteban A. Orellana and Andrea L. Kasinski, date: 11/05/2016, view: 36796, Q&A: 0
    [Abstract] The SRB assay has been used since its development in 1990 (Skehan et al., 1990) to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies (Vichai and Kirtikara, 2006). This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be ...
    TGFβ Stimulation Assay
    Author:  Emanuel Rognoni, date: 12/05/2014, view: 11449, Q&A: 0
    [Abstract] TGFβ is part of a growth factor superfamily which modulates cell growth, differentiation, adhesion, migration, ECM synthesis and apoptosis (Massague, 1998; Siegel and Massague, 2003). Free TGFβ binds to its high affinity TGFβ receptor, a receptor serine/threonine kinase, inducing phosphorylation of Smad2/3 which subsequently forms a complex with ...
    In vitro Regulatory T cells Differentiation From Naïve T Cells
    Authors:  Tomás Dalotto-Moreno, Gabriel A. Rabinovich and Marian Salatino, date: 03/20/2014, view: 17745, Q&A: 1
    [Abstract] In the past years, a subset of regulatory T cells (Tregs) expressing CD4, CD25 and the transcription factor FoxP3 has gained considerable attention as key regulators of T-cell tolerance and homeostasis (Sakaguchi, 2004). This population of T cells is specifically engaged in the maintenance of immune self-tolerance and the control of aberrant ...



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