Karyotype Analysis
Authors: Chun-Hung Chou and
Muh-Hwa Yang,
date: 05/20/2014,
view: 23328,
Q&A: 0
[Abstract] A chromosome is the structure that organizes DNA and protein in cells. It is a single piece of coiled DNA containing coding and non-coding sequences. Human cells have 23 pairs of chromosomes including 22 pairs of autosomes and one pair of sex chromosome, giving a total of 46 per cell. In tumor cells, chromosomal instability has been considered to ...
ImmunoFISH for Adherent Cultured Mammalian Cells
[Abstract] This protocol is optimized for immunoFISH staining of adherent cultured mammalian cells. It combines immunofluorescence for DNA damage response factors (e.g. 53BP1) and FISH against telomeric DNA.
Fluorescence in situ Hybridization to the Polytene Chromosomes of Anopheles Mosquitoes
[Abstract] Fluorescence in situ hybridization (FISH) is a method that uses a fluorescently labeled DNA probe for mapping the position of a genetic element on chromosomes. A DNA probe is prepared by incorporating Cy-3 or Cy-5 labeled nucleotides into DNA by nick-translation or a random primed labeling method. This protocol was used to map genes ...