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Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels

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Sleeping Beauty Transposon-based System for Rapid Generation of HBV-replicating Stable Cell Lines

Authors: Jin-Wei Zheng, Jia-Li Cao and Quan Yuan , date: 07/05/2018, view: 5127, Q&A: 0

[Abstract] The stable HBV-transfected cell lines, which based on stable integration of replication-competent HBV genome into hepatic cells, are widely used in basic research and antiviral drug evaluation against HBV. However, previous reported strategies to generate HBV-replicating cell lines, which primarily rely on random integration of exogenous DNA by ...

Preparation of Mitotic and Meiotic Metaphase Chromosomes from Young Leaves and Flower Buds of Coccinia grandis

Authors: Sangram Sinha, Kanika Karmakar, Ravi S. Devani, Jayeeta Banerjee, Rabindra K. Sinha and Anjan K. Banerjee, date: 04/05/2016, view: 11311, Q&A: 2

[Abstract] Somatic chromosomes are usually studied from the root tip cells of the plants for cytological investigations. In dioecious plant species like Coccinia grandis, it is very difficult to get meristematic root tip cells from the mature plants of the respective sex forms. In this report, young leaves of the respective sexual phenotypes were ...

Novel Method for Site-specific Induction of Oxidative DNA Damage to Study Recruitment of Repair Proteins to Heterochromatin and Euchromatin

Authors: Leizhen Wei, Satoshi Nakajima, Arthur S. Levine and Li Lan, date: 06/05/2014, view: 7728, Q&A: 0

[Abstract] ROS-induced DNA damage is repaired in living cells within a temporal and spatial context, and chromatin structure is critical to a consideration of DNA repair processes in situ. It’s well known that chromatin remodeling factors participate in many DNA damage repair pathways, indicating the importance of chromatin remodeling in ...

Karyotype Analysis

Authors: Chun-Hung Chou and Muh-Hwa Yang, date: 05/20/2014, view: 23328, Q&A: 0

[Abstract] A chromosome is the structure that organizes DNA and protein in cells. It is a single piece of coiled DNA containing coding and non-coding sequences. Human cells have 23 pairs of chromosomes including 22 pairs of autosomes and one pair of sex chromosome, giving a total of 46 per cell. In tumor cells, chromosomal instability has been considered to ...

Protocol for the Preparation of Arabidopsis Meiotic Chromosome Spreads and Fluorescent in situ Hybridization

Authors: Pablo Bolaños-Villegas, Xiaohui Yang, Christopher A. Makaroff and Guang-Yuh Jauh, date: 04/20/2014, view: 12781, Q&A: 0

[Abstract] This protocol is a more detailed version of previous protocols (Yang et al., 2011; Bolaños-Villegas et al., 2013) developed for the examination of meiotic chromosome spreads. Meiotic chromosome spreads are useful to determine the presence of defects in chromosome pairing and segregation. The protocol also describes how to perform ...

ImmunoFISH for Adherent Cultured Mammalian Cells

Authors: Francesca Rossiello, Marzia Fumagalli and Fabrizio d’Adda di Fagagna, date: 12/20/2013, view: 8877, Q&A: 0

[Abstract] This protocol is optimized for immunoFISH staining of adherent cultured mammalian cells. It combines immunofluorescence for DNA damage response factors (e.g. 53BP1) and FISH against telomeric DNA.

Fluorescence in situ Hybridization to the Polytene Chromosomes of Anopheles Mosquitoes

Authors: Ai Xia, Ashley Peery, Maryam Kamali, Jiangtao Liang, Maria V. Sharakhova and Igor V. Sharakhov, date: 08/20/2013, view: 10246, Q&A: 0

[Abstract] Fluorescence in situ hybridization (FISH) is a method that uses a fluorescently labeled DNA probe for mapping the position of a genetic element on chromosomes. A DNA probe is prepared by incorporating Cy-3 or Cy-5 labeled nucleotides into DNA by nick-translation or a random primed labeling method. This protocol was used to map genes ...

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