Arthur Levine Microbiology and Molecular Genetics, University of Pittsburgh, USA
1 protocol

Satoshi Nakajima Microbiology and Molecular Genetics, University of Pittsburgh, USA
1 protocol

Leizhen Wei Microbiology and Molecular Genetics, University of Pittsburgh, USA
1 protocol

Li Lan
  • Microbiology and Molecular Generits, University of Pittsburgh, USA
Research focus
  • Cell biology
  • 1 Author merit


Ph.D., Tohoku University, 2005

Current position

Assistant Professor, Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh

Publications (since 2003)

  1. Lan, L.*, Nakajima, S., Wei, L., Sun, L., Hsieh, C. L., Sobol, R. W., Bruchez, M., Van Houten, B., Yasui, A. and Levine, A. S. (2014). Novel method for site-specific induction of oxidative DNA damage reveals differences in recruitment of repair proteins to heterochromatin and euchromatin. Nucleic Acids Res 42(4): 2330-2345. (*Co-respondence)
  2. Nakajima, S.*, Lan, L.*, Wei, L., Hsieh, C. L., Rapic-Otrin, V., Yasui, A. and Levine, A. S. (2014). Ubiquitin-specific protease 5 is required for the efficient repair of DNA double-strand breaks. PLoS One 9(1): e84899. (*Co-respondence)
  3. Benitez, A., Yuan, F., Nakajima, S., Wei, L., Qian, L., Myers, R., Hu, J. J., Lan, L. and Zhang, Y. (2014). Damage-dependent regulation of MUS81-EME1 by Fanconi anemia complementation group A protein. Nucleic Acids Res 42(3): 1671-1683.
  4. Xu, L., Chen, Y.-C., Nakajima, S., Chong, J., Wang, L., Lan, L., Zhang, C. and Wang, D. (2014). A chemical probe targets DNA 5-formylcytosine sites and inhibits TDG excision, polymerases bypass, and gene expression. Chemical Science 5(2): 567-574.
  5. Wei, L., Nakajima, S., Hsieh, C. L., Kanno, S., Masutani, M., Levine, A. S., Yasui, A. and Lan, L*. (2013). Damage response of XRCC1 at sites of DNA single strand breaks is regulated by phosphorylation and ubiquitylation after degradation of poly(ADP-ribose). J Cell Sci 126(Pt 19): 4414-4423. (*Co-respondence)
  6. Lan, L., Nakajima, S., Kapetanaki, M. G., Hsieh, C. L., Fagerburg, M., Thickman, K., Rodriguez-Collazo, P., Leuba, S. H., Levine, A. S. and Rapic-Otrin, V. (2012). Monoubiquitinated histone H2A destabilizes photolesion-containing nucleosomes with concomitant release of UV-damaged DNA-binding protein E3 ligase. J Biol Chem 287(15): 12036-12049.
  7. Wei, L., Lan, L., Yasui, A., Tanaka, K., Saijo, M., Matsuzawa, A., Kashiwagi, R., Maseki, E., Hu, Y., Parvin, J. D., Ishioka, C. and Chiba, N. (2011). BRCA1 contributes to transcription-coupled repair of DNA damage through polyubiquitination and degradation of Cockayne syndrome B protein. Cancer Sci 102(10): 1840-1847.
  8. Horibata, K., Saijo, M., Bay, M. N., Lan, L., Kuraoka, I., Brooks, P. J., Honma, M., Nohmi, T., Yasui, A. and Tanaka, K. (2011). Mutant Cockayne syndrome group B protein inhibits repair of DNA topoisomerase I-DNA covalent complex. Genes Cells 16(1): 101-114.
  9. Lan, L., Ui, A., Nakajima, S., Hatakeyama, K., Hoshi, M., Watanabe, R., Janicki, S. M., Ogiwara, H., Kohno, T., Kanno, S. and Yasui, A. (2010). The ACF1 complex is required for DNA double-strand break repair in human cells. Mol Cell 40(6): 976-987.
  10. Asagoshi, K., Liu, Y., Masaoka, A., Lan, L., Prasad, R., Horton, J. K., Brown, A. R., Wang, X. H., Bdour, H. M., Sobol, R. W., Taylor, J. S., Yasui, A. and Wilson, S. H. (2010). DNA polymerase beta-dependent long patch base excision repair in living cells. DNA Repair (Amst) 9(2): 109-119.
  11. Wei, L., Lan, L., Hong, Z., Yasui, A., Ishioka, C. and Chiba, N. (2008). Rapid recruitment of BRCA1 to DNA double-strand breaks is dependent on its association with Ku80. Mol Cell Biol 28(24): 7380-7393.
  12. Hong, Z., Jiang, J., Hashiguchi, K., Hoshi, M., Lan, L. and Yasui, A. (2008). Recruitment of mismatch repair proteins to the site of DNA damage in human cells. J Cell Sci 121(Pt 19): 3146-3154.
  13. Hong, Z., Jiang, J., Lan, L., Nakajima, S., Kanno, S., Koseki, H. and Yasui, A. (2008). A polycomb group protein, PHF1, is involved in the response to DNA double-strand breaks in human cell. Nucleic Acids Res 36(9): 2939-2947.
  14. Kamath-Loeb, A. S., Lan, L., Nakajima, S., Yasui, A. and Loeb, L. A. (2007). Werner syndrome protein interacts functionally with translesion DNA polymerases. Proc Natl Acad Sci U S A 104(25): 10394-10399.
  15. Kanno, S., Kuzuoka, H., Sasao, S., Hong, Z., Lan, L., Nakajima, S. and Yasui, A. (2007). A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses. EMBO J 26(8): 2094-2103.
  16. Hirano, M., Yamamoto, A., Mori, T., Lan, L., Iwamoto, T. A., Aoki, M., Shimada, K., Furiya, Y., Kariya, S., Asai, H., Yasui, A., Nishiwaki, T., Imoto, K., Kobayashi, N., Kiriyama, T., Nagata, T., Konishi, N., Itoyama, Y. and Ueno, S. (2007). DNA single-strand break repair is impaired in aprataxin-related ataxia. Ann Neurol 61(2): 162-174.
  17. Saberi, A., Hochegger, H., Szuts, D., Lan, L., Yasui, A., Sale, J. E., Taniguchi, Y., Murakawa, Y., Zeng, W., Yokomori, K., Helleday, T., Teraoka, H., Arakawa, H., Buerstedde, J. M. and Takeda, S. (2007). RAD18 and poly(ADP-ribose) polymerase independently suppress the access of nonhomologous end joining to double-strand breaks and facilitate homologous recombination-mediated repair. Mol Cell Biol 27(7): 2562-2571.
  18. Yoshimura, M., Kohzaki, M., Nakamura, J., Asagoshi, K., Sonoda, E., Hou, E., Prasad, R., Wilson, S. H., Tano, K., Yasui, A., Lan, L., Seki, M., Wood, R. D., Arakawa, H., Buerstedde, J. M., Hochegger, H., Okada, T., Hiraoka, M. and Takeda, S. (2006). Vertebrate POLQ and POLbeta cooperate in base excision repair of oxidative DNA damage. Mol Cell 24(1): 115-125.
  19. Nakajima, S., Lan, L., Kanno, S., Usami, N., Kobayashi, K., Mori, M., Shiomi, T. and Yasui, A. (2006). Replication-dependent and -independent responses of RAD18 to DNA damage in human cells. J Biol Chem 281(45): 34687-34695.
  20. Karmakar, P., Seki, M., Kanamori, M., Hashiguchi, K., Ohtsuki, M., Murata, E., Inoue, E., Tada, S., Lan, L., Yasui, A. and Enomoto, T. (2006). BLM is an early responder to DNA double-strand breaks. Biochem Biophys Res Commun 348(1): 62-69.
  21. Lan, L., Nakajima, S., Komatsu, K., Nussenzweig, A., Shimamoto, A., Oshima, J. and Yasui, A. (2005). Accumulation of Werner protein at DNA double-strand breaks in human cells. J Cell Sci 118(Pt 18): 4153-4162.
  22. Braithwaite, E. K., Kedar, P. S., Lan, L., Polosina, Y. Y., Asagoshi, K., Poltoratsky, V. P., Horton, J. K., Miller, H., Teebor, G. W., Yasui, A. and Wilson, S. H. (2005). DNA polymerase lambda protects mouse fibroblasts against oxidative DNA damage and is recruited to sites of DNA damage/repair. J Biol Chem 280(36): 31641-31647.
  23. Wilson, T. M., Vaisman, A., Martomo, S. A., Sullivan, P., Lan, L., Hanaoka, F., Yasui, A., Woodgate, R. and Gearhart, P. J. (2005). MSH2-MSH6 stimulates DNA polymerase eta, suggesting a role for A:T mutations in antibody genes. J Exp Med 201(4): 637-645.
  24. Okano, S., Lan, L., Tomkinson, A. E. and Yasui, A. (2005). Translocation of XRCC1 and DNA ligase IIIalpha from centrosomes to chromosomes in response to DNA damage in mitotic human cells. Nucleic Acids Res 33(1): 422-429.
  25. Lan, L., Nakajima, S., Oohata, Y., Takao, M., Okano, S., Masutani, M., Wilson, S. H. and Yasui, A. (2004). In situ analysis of repair processes for oxidative DNA damage in mammalian cells. Proc Natl Acad Sci U S A 101(38): 13738-13743.
  26. Nakajima, S., Lan, L., Kanno, S., Takao, M., Yamamoto, K., Eker, A. P. and Yasui, A. (2004). UV light-induced DNA damage and tolerance for the survival of nucleotide excision repair-deficient human cells. J Biol Chem 279(45): 46674-46677.
  27. Lan, L., Hayashi, T., Rabeya, R. M., Nakajima, S., Kanno, S., Takao, M., Matsunaga, T., Yoshino, M., Ichikawa, M., Riele, H., Tsuchiya, S., Tanaka, K. and Yasui, A. (2004). Functional and physical interactions between ERCC1 and MSH2 complexes for resistance to cis-diamminedichloroplatinum(II) in mammalian cells. DNA Repair (Amst) 3(2): 135-143.
  28. Okano, S., Lan, L., Caldecott, K. W., Mori, T. and Yasui, A. (2003). Spatial and temporal cellular responses to single-strand breaks in human cells. Mol Cell Biol 23(11): 3974-3981.
1 Protocol published
Novel Method for Site-specific Induction of Oxidative DNA Damage to Study Recruitment of Repair Proteins to Heterochromatin and Euchromatin
Authors:  Leizhen Wei, Satoshi Nakajima, Arthur S. Levine and Li Lan, date: 06/05/2014, view: 8833, Q&A: 0
ROS-induced DNA damage is repaired in living cells within a temporal and spatial context, and chromatin structure is critical to a consideration of DNA repair processes in situ. It’s well known that chromatin remodeling factors participate ...
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