Guang-Yuh Jauh Institute of Plant and Microbial Biology, Academia Sinica, Taiwan
3 protocols

Xiaohui Yang Department of Chemistry and Biochemistry, Miami University, USA
1 protocol

Pablo Bolanos-Villegas University of Costa Rica
1 protocol

Christopher A. Makaroff
  • Department of Chemistry and Biochemistry, Miami University, USA
Research focus
  • Plant science
  • 1 Author merit


Ph.D. in Biochemistry, Purdue University, 1986

Current position

Professor, Department of Chemistry and Biochemistry, Miami University
Associate Dean, College of Arts and Science, Miami University (2012-present)


Research papers (since 2008)
(*Graduate student and **Undergraduate student co-authors)

  1. Yuan, L., Yang, X., **Auman, D. and Makaroff, C. A. (2014). Expression of Epitope-Tagged SYN3 Cohesin Proteins Can Disrupt Meiosis in Arabidopsis. J Genet Genomics 41(3): 153-164.
  2. *Devanathan, S., Erban, A., **Perez-Torres Jr, R., Kopka, J. and Makaroff, C. A. (2013). Arabidopsis thaliana Glyoxalase 2-1 is required during abiotic stress but is not essential under normal plant growth. Plos One (Provisional acceptance)
  3. *De, K., **Sterle, L., **Krueger, L., Yang, X. and Makaroff, C. A. (2013). Arabidopsis WAPL is Essential for the Prophase Removal of Cohesin During Meiosis. Plos Genetics (Under revision)
  4. Bolaños-Villegas, P., Yang, X., Wang, H. J., Juan, C. T., Chuang, M. H., Makaroff, C. A. and Jauh, G. Y. (2013). Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis. Plant J 75(6): 927-940.
  5. Holdorf, M. M., Owen, H. A., *Lieber, S. R., Yuan, L., **Adams, N., Dabney-Smith, C. and Makaroff, C. A. (2012). Arabidopsis ETHE1 encodes a sulfur dioxygenase that is essential for embryo and endosperm development. Plant Physiol 160(1): 226-236.
  6. Yuan, L., Yang, X., **Ellis, J. L., **Fisher, N. M. and Makaroff, C. A. (2012). The Arabidopsis SYN3 cohesin protein is important for early meiotic events. Plant J 71(1): 147-160.
  7. Yang, X., *Boateng, K. A., Yuan, L., Wu, S., Baskin, T. I. and Makaroff, C. A. (2011). The radially swollen 4 separase mutation of Arabidopsis thaliana blocks chromosome disjunction and disrupts the radial microtubule system in meiocytes. PLoS One 6(4): e19459.
  8. Yuan, L., Yang, X. and Makaroff, C. A. (2011). Plant cohesins, common themes and unique roles. Curr Protein Pept Sci 12(2): 93-104.
  9. *Limphong, P., **Adams, N. E., *Rouhier, M. F., **McKinney, R. M., **Naylor, M., Bennett, B., Makaroff, C. A. and Crowder, M. W. (2010). Converting GLX2-1 into an active glyoxalase II. Biochemistry 49(37): 8228-8236.
  10. Jiang, L., Yuan, L., Xia, M. and Makaroff, C. A. (2010). Proper levels of the Arabidopsis cohesion establishment factor CTF7 are essential for embryo and megagametophyte, but not endosperm, development. Plant Physiol 154(2): 820-832.
  11. *Limphong, P., **McKinney, R. M., **Adams, N. E., Makaroff, C. A., Bennett, B. and Crowder, M. W. (2010). The metal ion requirements of Arabidopsis thaliana Glx2-2 for catalytic activity. J Biol Inorg Chem 15(2): 249-258.
  12. *Limphong, P., *Nimako, G., Thomas, P. W., Fast, W., Makaroff, C. A. and Crowder, M. W. (2009). Arabidopsis thaliana mitochondrial glyoxalase 2-1 exhibits beta-lactamase activity. Biochemistry 48(36): 8491-8493.
  13. Yang, X., *Boateng, K. A., Strittmatter, L., **Burgess, R. and Makaroff, C. A. (2009). Arabidopsis separase functions beyond the removal of sister chromatid cohesion during meiosis. Plant Physiol 151(1): 323-333.
  14. *Limphong, P., **McKinney, R. M., **Adams, N. E., Bennett, B., Makaroff, C. A., Gunasekera, T. and Crowder, M. W. (2009). Human glyoxalase II contains an Fe(II)Zn(II) center but is active as a mononuclear Zn(II) enzyme. Biochemistry 48(23): 5426-5434.
  15. *Limphong, P., Crowder, M. W., Bennett, B. and Makaroff, C. A. (2009). Arabidopsis thaliana GLX2-1 contains a dinuclear metal binding site, but is not a glyoxalase 2. Biochem J 417(1): 323-330.
  16. *Holdorf, M. M., Bennett, B., Crowder, M. W. and Makaroff, C. A. (2008). Spectroscopic studies on Arabidopsis ETHE1, a glyoxalase II-like protein. J Inorg Biochem 102(9): 1825-1830.
  17. *Boateng, K. A., Yang, X., Dong, F., Owen, H. A. and Makaroff, C. A. (2008). SWI1 is required for meiotic chromosome remodeling events. Mol Plant 1(4): 620-633.

Book chapters

  1. Yang, X., Yuan, L. and Makaroff, C. A. (2013). Immunolocalization Methods for Visualizing Meiotic Proteins in Arabidopsis thaliana. Plant Meiosis, Methods in Molecular Biology 990. In: Pawlowski, W., Grelon, M. and Armstrong, S. (eds). Humana Press, 109-118.
  2. Makaroff, C. A. (1997). Cytoplasmic Male Sterility: Molecular Studies, In Recent Advances in Oilseed Brassicas. In: Kalia, H. R. and Gupta, S. K. (eds). Ludhiana, 92-111.
  3. Makaroff, C. A. (1995). CMS of Brassica, In Advances in Cellular and Molecular Biology of Plants volume 2: Molecular Biology of the Mitochondria. In: Levings, C. S.and Vasil, I. K. (eds). 515-555.
  4. Palmer, J. D., Makaroff, C. A., Apel, I. J. and Shirzadigan, M. (1990). Fluid Structure of Plant Mitochondrial Genomes: Evolutionary and Functional Implications, UCLA Symposia on Molecular and Cellular Biology. In: Clegg, M. and Brian, G. S. O. (eds). Alan Liss Inc., 85-96.
1 Protocol published
Protocol for the Preparation of Arabidopsis Meiotic Chromosome Spreads and Fluorescent in situ Hybridization
Authors:  Pablo Bolaños-Villegas, Xiaohui Yang, Christopher A. Makaroff and Guang-Yuh Jauh, date: 04/20/2014, view: 14435, Q&A: 0
This protocol is a more detailed version of previous protocols (Yang et al., 2011; Bolaños-Villegas et al., 2013) developed for the examination of meiotic chromosome spreads. Meiotic chromosome spreads are useful to determine the ...
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