Cell Biology


    Protocols in Current Issue
    High Throughput Traction Force Microscopy for Multicellular Islands on Combinatorial Microarrays
    Authors:  Ian C. Berg and Gregory H. Underhill, date: 11/05/2019, view: 2390, Q&A: 0
    [Abstract] The composition and mechanical properties of the cellular microenvironment along with the resulting distribution of cellular devolved forces can affect cellular function and behavior. Traction Force Microscopy (TFM) provides a method to measure the forces applied to a surface by adherent cells. Numerous TFM systems have been described in ...
    An Image Analysis Pipeline to Quantify Emerging Cracks in Materials or Adhesion Defects in Living Tissues
    Authors:  Stéphane Verger, Guillaume Cerutti and Olivier Hamant, date: 10/05/2018, view: 3297, Q&A: 0
    [Abstract] Microcracks in materials reflect their mechanical properties. The quantification of the number or orientation of such cracks is thus essential in many fields, including engineering and geology. In biology, cracks in soft tissues can reflect adhesion defects, and the analysis of their pattern can help to deduce the magnitude and orientation of ...
    Estimation of Stomatal Aperture in Arabidopsis thaliana Using Silicone Rubber Imprints
    Authors:  Telma E. Scarpeci, María I. Zanor and Estela M. Valle, date: 06/20/2017, view: 7725, Q&A: 2
    [Abstract] Estimation of stomatal aperture using low viscosity silicone-base impression material has the advantage of working with the whole leaf. The developmental stage and the environment strongly affect the stomatal aperture. Therefore, it is mandatory to have accurate estimations of the stomatal aperture of intact leaves under different situations. With ...
    Measurement of Mechanical Tension at cell-cell junctions using two-photon laser ablation
    Authors:  Xuan Liang, Magdalene Michael and Guillermo A. Gomez, date: 12/20/2016, view: 7108, Q&A: 0
    [Abstract] The cortical actomyosin cytoskeleton is found in all non-muscle cells where a key function is to control mechanical force (Salbreux et al., 2012). When coupled to E-cadherin cell-cell adhesion, cortical actomyosin generates junctional tension that influences many aspects of tissue function, organization and morphogenesis (Lecuit and Yap, ...
    Analysis of Myosin II Minifilament Orientation at Epithelial Zonula Adherens
    Authors:  Magdalene Michael, Xuan Liang and Guillermo A. Gomez, date: 12/05/2016, view: 6305, Q&A: 0
    [Abstract] Non-muscle myosin II (NMII) form bipolar filaments, which bind F-actin to exert cellular contractility during physiological processes (Vicente-Manzanares et al., 2009). Using a combinatorial approach to fluorescently label both N- and C-termini of the NMII heavy chain, recent works have demonstrated the ability to visualize NMII bipolar ...
    In vitro Microtubule Binding Assay and Dissociation Constant Estimation
    Authors:  Christopher Kesten, René Schneider and Staffan Persson, date: 03/20/2016, view: 10847, Q&A: 0
    [Abstract] Microtubules (MTs) support an astonishing set of versatile cellular functions ranging from cell division, vesicle transport, and cell and tissue morphogenesis in various organisms. This versatility is in large mediated by MT-associated proteins (MAPs). The neuronal MAP Tau, for example, is stabilizing MTs in axons of the vertebrate nervous system ...
    Imaging and Measurement of Nanomechanical Properties within Primary Xylem Cell Walls of Broadleaves
    Authors:  Jaroslav Ďurkovič, Monika Kardošová and Rastislav Lagaňa, date: 12/20/2014, view: 9726, Q&A: 0
    [Abstract] A technique of atomic force microscopy (AFM) called PeakForce quantitative nanomechanical mapping (PeakForce QNM) is an efficient tool for the quantitative mechanobiological imaging of fibrillar aggregate, human epidermal cell and woody plant cell wall topography (Sweers et al., 2011; Heu et al., 2012; Ďurkovič et al., ...
    Assay for Adherence of Vibrio cholerae to Eukaryotic Cell Lines
    Authors:  Amit K. Dey, Abha Bhagat and Rukhsana Chowdhury, date: 04/20/2014, view: 9057, Q&A: 0
    [Abstract] Vibrio cholerae (V. cholerae) colonizes the intestinal epithelium and secretes cholera toxin (CT), a potent enterotoxin that causes severe fluid loss characteristic of the disease cholera. V. cholerae is a non-invasive Gram-negative bacterium that adheres to intestinal cells as well as a variety of different cell types. ...
    Adhesion of Moraxella catarrhalis to Respiratory Tract Epithelial Cells
    Authors:  Stefan P.W. de Vries and Hester J. Bootsma, date: 11/05/2013, view: 7636, Q&A: 0
    [Abstract] Moraxella catarrhalis is a human-restricted pathogen that is responsible for respiratory tract infections such as childhood otitis media (OM) and exacerbation of chronic obstructive pulmonary disease (COPD) in adults. Successful colonization and infection by M. catarrhalis depends on its ability to attach to the respiratory tract ...
    Analyzing Inhibitory Effects of Reagents on Mycoplasma Gliding and Adhesion
    Authors:  Taishi Kasai and Makoto Miyata, date: 07/20/2013, view: 9394, Q&A: 0
    [Abstract] Dozens of Mycoplasma species bind to solid surfaces and glide in the direction of the membrane protrusion at a pole. In gliding, Mycoplasma legs catch, pull and release sialylated oligosaccharides fixed on a solid surface. The analyses of inhibitory effects of sialylated compounds on gliding of Mycoplasma can determine ...

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