Organelle-associated rRNA Degradation
Authors: Jinliang Huang and
Geng Wang,
date: 06/05/2019,
view: 2672,
Q&A: 0
[Abstract] Cytosolic rRNAs are highly dynamic and can be degraded under conditions such as apoptosis, starvation and magnesium depletion. The degradation is also related to their specific localization, as fractions of cytosolic ribosomes are localized on the surfaces of intracellular organelles, such as endoplasmic reticulum (ER) and mitochondria. Such ...
Improved Mammalian Mitochondrial RNA Isolation
Authors: Jinliang Huang and
Geng Wang,
date: 06/05/2019,
view: 3885,
Q&A: 0
[Abstract] Mitochondria have two sets of RNAs. One is encoded in mitochondrial genome, and the other that consists of imported RNAs within mitochondria and cytosolic RNAs associated with mitochondrial outer membrane is encoded in the nucleus. These mitochondrial RNAs play important roles in mitochondrion biosynthesis and signaling in and out of mitochondria. ...
Ca2+ Measurements in Mammalian Cells with Aequorin-based Probes
[Abstract] Aequorin is a Ca2+ sensitive photoprotein suitable to measure intracellular Ca2+ transients in mammalian cells. Thanks to recombinant cDNAs expression, aequorin can be specifically targeted to various subcellular compartments, thus allowing an accurate measurement of Ca2+ uptake and release of different ...
Mouse Liver Mitochondria Isolation, Size Fractionation, and Real-time MOMP Measurement
[Abstract] The mitochondrial pathway of apoptosis involves a complex interplay between dozens of proteins and lipids, and is also dependent on the shape and size of mitochondria. The use of cellular models in past studies has not been ideal for investigating how the complex multi-factor interplay regulates the molecular mechanisms of mitochondrial outer ...
Isolation of Mitochondria from Potato Tubers
Authors: Jesper F. Havelund,
Fernanda Salvato,
Mingjie Chen,
R.S.P. Rao,
Adelina Rogowska-Wrzesinska,
Ole N. Jensen,
David R. Gang,
Jay J. Thelen and
Ian Max Møller,
date: 09/05/2014,
view: 14087,
Q&A: 2
[Abstract] One way to study the function of plant mitochondria is to extract them from plant tissues in an uncontaminated, intact and functional form. The reductionist assumption is that the components present in such a preparation and the in vitro measurable functions or activities reliably reflect the in vivo properties of the organelle ...