Live Confocal Imaging of Brachypodium Spikelet Meristems
Author: Devin Lee O'Connor,
date: 09/20/2018,
view: 2883,
Q&A: 0
[Abstract] Live confocal imaging of fluorescent reporters and stains in plant meristems provides valuable measurements of gene expression, protein dynamics, cell polarity, cell division, and growth. The spikelet meristem in the grass Brachypodium distachyon (Brachypodium) is well suited to live imaging because of the ease of dissection, small ...
A Modified Approach for Axenic Cultivation of Spores of Fern Adiantum capillus-veneris L. with High Germination Rate
Authors: Yuan Ya-Ning,
Yi Shu,
Jin Wan-Ting and
Fang Yu-Han,
date: 07/05/2018,
view: 2547,
Q&A: 0
[Abstract] Spores are the primary way of spread and reproduction for ferns, a clade of seed-free vascular plants. However, no detailed protocol for ferns spore cultivation has been reported yet. Here we provide a modified approach for axenic cultivation of fern Adiantum capillus-veneris L., based on Cao’s and Li’s method (Cao, et al., 2010; ...
Comprehensive Methods for Leaf Geometric Morphometric Analyses
Authors: Laura L. Klein and
Harlan T. Svoboda,
date: 05/05/2017,
view: 9680,
Q&A: 0
[Abstract] Leaf morphometrics are used frequently by several disciplines, including taxonomists, systematists, developmental biologists, morphologists, agronomists, and plant breeders to name just a few. Leaf shape is highly variable and can be used for identifying species or genotypes, developmental patterning within and among individuals, assessing plant ...
Automated Tracking of Root for Confocal Time-lapse Imaging of Cellular Processes
[Abstract] Here we describe a protocol that enables to automatically perform time-lapse imaging of growing root tips for several hours. Plants roots expressing fluorescent proteins or stained with dyes are imaged while they grow using automatic movement of the microscope stage that compensates for root growth and allows to follow a given region of the root ...
In vitro Floral Induction of Cuscuta reflexa
Authors: Priyanka Das and
Santilata Sahoo,
date: 01/20/2017,
view: 5261,
Q&A: 0
[Abstract] Floral initiation and development in the angiosperms is the essential step on which the yield of the plant depends. Sometimes external climate or any abiotic stress hinders the floral initiation and ultimately affect the plant yield. Hence, in vitro floral induction and development can help to overcome the external climatic factor. ...
Shoot Apical Meristem Size Measurement
[Abstract] The shoot apical meristem (SAM) is a collection of cells that continuously renew themselves by cell division and also provide cells to newly developing organs. It has been known that CLAVATA (CLV) 3 peptide regulates a transcription factor WUSCHEL (WUS) to keep numbers of undifferentiated cells constant and maintain the size of the SAM. The ...