Cell Biology

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    Protocols in Current Issue
    In-vitro GLP-1 Release Assay Using STC-1 Cells
    Authors:  Liu Qi, Tan Shuai, Jia Da, Burstein Ezra and Sifuentes-Dominguez Luis, date: 08/20/2020, view: 618, Q&A: 0
    [Abstract] Enteroendocrine cells (EECs) are known chemosensors in the gastrointestinal (GI) epithelium. They release a diversity of gut hormones in response to various stimuli. Here, we report an in-vitro assay to measure GLP-1 release from cultured murine EEC’s under fatty acid stimulation.
    Method for Prolonged Incubation of Brain Slices
    Authors:  Orsolya Kekesi and Yossi Buskila, date: 07/20/2020, view: 440, Q&A: 0
    [Abstract] Slices of neuronal tissue maintain a high degree of topographical and functional properties of neurons and glia and therefore are extensively used for measurements of neuronal activity at the molecular, cellular and network levels. However, the lifespan of slice preparations is narrow, averaging of 6-8 hours. Moreover, the average viability of ...
    Application of Mechanical Forces on Drosophila Embryos by Manipulation of Microinjected Magnetic Particles
    Authors:  Arturo D’Angelo and Jérôme Solon, date: 05/05/2020, view: 898, Q&A: 0
    [Abstract] Cells generate mechanical forces to shape tissues during morphogenesis. These forces can activate several biochemical pathways and trigger diverse cellular responses by mechano-sensation, such as differentiation, division, migration and apoptosis. Assessing the mechano-responses of cells in living organisms requires tools to apply controlled local ...
    Ex vivo Culture and Lentiviral Transduction of Benign Prostatic Hyperplasia (BPH) Samples
    Authors:  Urszula Lucja McClurg, Stuart R McCracken, Lisa Butler, Karl T Riabowol and Olivier Binda, date: 11/05/2018, view: 2374, Q&A: 0
    [Abstract] To assess oncogenic potential, classical transformation assays are based on cell line models. However, cell line based models do not reflect the complexity of human tissues. We thus developed an inducible expression system for gene expression in ex vivo human tissues, which maintain native tissue architecture, such as epithelia and ...



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