Extraction and Quantification of Poly P, Poly P Analysis by Urea-PAGE
Author: Maria R. Gomez Garcia,
date: 05/05/2014,
view: 8604,
Q&A: 1
[Abstract] Inorganic polyphosphate (poly P) molecules, linear chains containing hundreds of orthophosphate (Pi) residues linked by high-energy phosphoanhydride bonds are abundant in every cell in nature. These molecules are widely distributed among bacteria, including key pathogens, and eukaryotes, poly P is present in organelles, including nuclei, ...
Quantification of Total and Soluble Inorganic Phosphate
Authors: Vicki Knowles and
William Plaxton,
date: 09/05/2013,
view: 13983,
Q&A: 0
[Abstract] A simple, rapid, and sensitive colorimetric microassay for inorganic phosphate (Pi) relies upon the absorption at 660 nm of a molybdenum blue complex that forms upon reduction of an ammonium molybdate-Pi complex in acid. The method for determination of total Pi uses plant tissues that have been ashed at 500 °C, whereas quantification of soluble Pi ...