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William Plaxton
Department of Biology, Queen’s University, Canada
3 protocols
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Vicki Knowles
Department of Biology, Queen's University, Canada
Research focus
Plant science
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2
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2 Protocols published
Quantification of Total and Soluble Inorganic Phosphate
Authors:
Vicki Knowles
and
William Plaxton
,
date:
09/05/2013,
view:
14102,
Q&A:
0
A simple, rapid, and sensitive colorimetric microassay for inorganic phosphate (Pi) relies upon the absorption at 660 nm of a molybdenum blue complex that forms upon reduction of an ammonium molybdate-Pi complex in acid. The method for determination ...
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Protein Extraction, Acid Phosphatase Activity Assays, and Determination of Soluble Protein Concentration
Authors:
Vicki Knowles
and
William Plaxton
,
date:
09/05/2013,
view:
21110,
Q&A:
0
Acid phosphatases (APases) catalyze the hydrolysis of inorganic phosphate (Pi) from a broad range of Pi-monoesters with an acidic pH optimum. The liberated Pi is reassimilated into cellular metabolism via mitochondrial or chloroplastic ATP synthases ...
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