Cancer Biology


    Protocols in Current Issue
    Triacylglycerol Measurement in HeLa Cells
    Authors:  Ximing Du and Hongyuan Yang, date: 12/20/2020, view: 2078, Q&A: 0

    Lipid droplets store triacylglycerols (triglycerides) and sterol esters to regulate lipid and energy homeostasis. Triacylglycerol measurement is often performed during the investigation of lipid droplet formation and growth. This protocol describes a reliable method using a fluorometric lipid quantification kit to measure triacylglycerols

    Measurement of the Intracellular Calcium Concentration with Fura-2 AM Using a Fluorescence Plate Reader
    Authors:  Magdiel Martínez, Namyr A. Martínez and Walter I. Silva, date: 07/20/2017, view: 29073, Q&A: 1
    [Abstract] Intracellular calcium elevation triggers a wide range of cellular responses. Calcium responses can be affected or modulated by membrane receptors mutations, localization, exposure to agonists/antagonists, among others (Burgos et al., 2007; Martínez et al., 2016). Changes in intracellular calcium concentration can be measured ...
    Metabolic Assays for Detection of Neutral Fat Stores
    Authors:  Jan M. Baumann, Leila Kokabee, Xianhiu Wang, Yan Sun, Jason Wong and Douglas S. Conklin, date: 06/20/2015, view: 13700, Q&A: 1
    [Abstract] Lipid droplets (LDs) are ubiquitous intracellular structures whose formation, growth, and maintenance are highly regulated (Wang et al., 2013; Ranall et al., 2011; Goodman, 2009). Lipid metabolism and droplet dynamics are of considerable interest to agriculture, biofuel production, viral pathology, nutrition, and cancer biology ...
    Bone Resorption Assay
    Authors:  Carina Scholtysek, Gerhard Krönke and Georg Schett, date: 07/20/2014, view: 15150, Q&A: 0
    [Abstract] The Bone resorption assay provides an easy to use protocol for quantitatively measuring in vitro osteoclast-mediated bone resorption. Osteoclasts can be seeded onto the bone slices and formation of resorption pits can be quantified via toluidinblue staining (Scholtysek et al., 2013).
    Lysosomal Stability Assay
    Authors:  Nikolaj H. T. Petersen, Thomas Kirkegaard and Marja Jäättelä, date: 06/20/2014, view: 15705, Q&A: 0
    [Abstract] This assay makes use of the dye Acridine Orange (AO) to determine the stability of lysosomes in living cells upon exposure to a confocal microscope laser.

    AO is a lipophilic amine that readily diffuses into cells (Figure 1). Inside the cell it enters the acidic lysosomal compartment where it is protonated and sequestered, shifting its ...
    Homologous Recombination Assay
    Authors:  Yvan Canitrot and Didier Trouche, date: 09/20/2013, view: 16986, Q&A: 0
    [Abstract] Repair of double strand break by homologous recombination was examined using U2OS cells or RG37 cells harbouring specific substrate developed by Puget et al. (2005) and Dumay et al. (2006), respectively, to measure the repair of DNA double strand breaks by homologous recombination. The substrate is composed of two inactive copies ...

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