Cancer Biology


    Protocols in Current Issue
    Image-based Quantification of Macropinocytosis Using Dextran Uptake into Cultured Cells
    Authors:  Anh H. Le and Laura M. Machesky, date: 04/05/2022, view: 519, Q&A: 0

    Macropinocytosis is an evolutionarily conserved process, which is characterized by the formation of membrane ruffles and the uptake of extracellular fluid. We recently demonstrated a role for CYFIP-related Rac1 Interactor (CYRI) proteins in macropinocytosis. High-molecular weight dextran (70kDa or higher) has generally been used as a marker for

    Spherical Invasion Assay: A Novel Method to Measure Invasion of Cancer Cells
    [Abstract] The invasion of tumor cells into the neighboring blood vessels and lymph nodes is a vital step for distant metastasis. Traditionally, the invasive activity of growth factors (or the anti-invasive activity of drugs) is measured with the Boyden chamber assay. However, this assay has a few disadvantages like poor physiological relevance of transwell ...
    Studying Chemotactic Migration in Dunn Chamber: An Example Applied to Adherent Cancer Cells
    Authors:  Khedidja Benseddik and Kossay Zaoui, date: 02/05/2022, view: 965, Q&A: 0

    Cell migration is a vital process in the development of multicellular organisms. When deregulated, it is involved in many diseases such as inflammation and cancer metastisation. Some cancer cells could be stimulated using chemoattractant molecules, such as growth factor Heregulin β1. They respond to the attractant or repellent gradients

    Imaging of Human Cancer Cells in 3D Collagen Matrices
    Authors:  Karin Pfisterer, Brooke Lumicisi and Maddy Parsons, date: 01/20/2021, view: 3558, Q&A: 0

    Research on cell migration and interactions with the extracellular matrix (ECM) was mostly focused on 2D surfaces in the past. Many recent studies have highlighted differences in migratory behaviour of cells on 2D surfaces compared to complex cell migration modes in 3D environments. When embedded in 3D matrices, cells constantly sense the

    Single Cell Migration Assay Using Human Breast Cancer MDA-MB-231 Cell Line
    Authors:  David M. Gau and Partha Roy, date: 04/20/2020, view: 4570, Q&A: 0
    [Abstract] Cell migration is a fundamental cellular process that plays a crucial role in many physioglogical and pathological processes such as wound healing or cancer metastasis. Many assays have been developed to examine cell migration, such as the wound healing or scratch assay, Boyden Chamber or transwell assay, and the method we will describe here, ...
    Analysis of Random Migration of Cancer Cells in 3D
    Authors:  Sai P. Visweshwaran and Alexis Gautreau, date: 01/05/2020, view: 4173, Q&A: 1
    [Abstract] The ability of cancer cells to migrate through a complex three-dimensional (3D) environment is a hallmark event of cancer metastasis. Therefore, an in vitro migration assay to evaluate cancer cell migration in a 3D setting is valuable to examine cancer progression. Here, we describe such a simple migration assay in a 3D ...
    The Chick Embryo Chorioallantoic Membrane as an in vivo Model to Study Metastasis
    Authors:  Piero Crespo and Berta Casar, date: 10/20/2016, view: 19234, Q&A: 0
    [Abstract] Metastasis is a complex process that includes several steps: neoplastic progression, angiogenesis, cell migration and invasion, intravasation into nearby blood vessels, survival in the circulatory system, extravasation followed by homing into distant tissues, the formation of micrometastases, and finally the growth into macroscopic secondary ...
    In vitro Tumor Cell Migration Assay Using ThinCertsTM (Transwells)
    Author:  Marc A. Schneider, date: 06/05/2016, view: 16095, Q&A: 0
    [Abstract] The high migration rate of tumor cells often results in poor prognosis for the survival of the patients. Here, we describe a protocol to measure the migration of cells using a quantitative assay. The relative tumor cell migration was measured using ThinCertsTM cell culture inserts and a lactate dehydrogenase (LDH) assay to quantify the ...
    Colon Cancer-associated Fibroblast Establishment and Culture Growth
    [Abstract] Cancer-associated fibroblasts (CAFs) are one of the major players in tumor-stroma crosstalk. Findings in experimental studies suggest important roles for CAFs in regulation of tumor growth, metastasis and drug response (Hanahan and Coussens, 2012). Furthermore, their clinical relevance is supported by new findings from tumor analyses, ...
    In vitro Flow Adhesion Assay for Analyzing Shear-resistant Adhesion of Metastatic Cancer Cells to Endothelial Cells
    Authors:  Shin-Ae Kang, Sandra Bajana and Takemi Tanaka, date: 02/20/2016, view: 12259, Q&A: 1
    [Abstract] Hematogenous metastasis is a primary cause of mortality from metastatic cancer. The shear-resistant adhesion of circulating tumor cells to the vascular endothelial cell surface under blood flow is an essential step in cell extravasation and further tissue invasion. This is similar to a process exploited by leukocytes for adhesion to inflamed blood ...

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