Sulfatase Assay to Determine Influence of Plants on Microbial Activity in Soil
[Abstract] Sulfatase activity is often used as a measure of the activity of soil microorganisms. It is thus a suitable tool to investigate the response of microbes to plants. Here we present a method to determine the influence of various Arabidopsis genotypes on the function of soil microbiota using the sulfatase as a quantitative measure. We grew ...
Determination of Mn Concentrations in Synechocystis sp. PCC6803 Using ICP-MS
[Abstract] Manganese (Mn) is an essential micronutrient for all photoautotrophic organisms. Two distinct pools of Mn have been identified in the cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis), with 80% of the Mn residing in the periplasm and 20% in cytoplasm and thylakoid lumen (Keren et al., 2002). In this protocol, we ...
Determination of Oxygen Respiration Rates in Wetted Developmentally Arrested Spores of Streptomyces Species
Authors: Marco Fischer,
Dörte Falke and
R. Gary Sawers,
date: 06/20/2014,
view: 7898,
Q&A: 0
[Abstract] Streptomyces species produce spores, which, while not as robust as endospores of Bacillus or Clostridium species, are capable of surviving for months or even years (Hopwood, 2006). During this time these spores remain viable, surviving by slowly degrading internal stores of carbon compounds, such as the carbohydrate ...
Transport Assays in Aspergillus nidulans
Authors: Emilia Krypotou and
George Diallinas,
date: 11/20/2013,
view: 7674,
Q&A: 0
[Abstract] Transport assays allow the direct kinetic analysis of a specific transporter by measuring apparent Km and Vmax values, and permit the characterization of substrate specificity profiles through competition assays. In this protocol, we describe a rapid and easy method for performing uptake assays in the model ...
Choline Uptake Assay in Bacterial Cells
[Abstract] Choline is a methylated nitrogen compound that is widespread in nature. It is a precursor of several metabolites that perform numerous biological functions and it is predominantly used for the synthesis of essential lipid components of the cell membranes. Since there is no evidence that prokariotes can synthesize choline de novo and ...