Bio-protocol - Improve Research Reproducibility

An in vitro Coupled Assay for PEPC with Control of Bicarbonate Concentration

Authors: Nicholas R. Moody, Chatawal Phansopal and James D. Reid, date: 12/20/2021, view: 799, Q&A: 0

[Abstract]

Phosphoenolpyruvate carboxylase (PEPC) catalyzes a critical step in carbon metabolism in plants and bacteria, the irreversible reaction between bicarbonate and phosphoenolpyruvate to produce the C4 compound oxaloacetate. This enzyme is particularly important in the context of C4 photosynthesis, where it is the initial carbon-fixing enzyme. Many

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In vitro Assessment of Pathogen Effector Binding to Host Proteins by Surface Plasmon Resonance

Authors: Marina Franceschetti, Mark J. Banfield, Clare E. M. Stevenson and Juan Carlos De la Concepcion, date: 07/05/2020, view: 2869, Q&A: 0

[Abstract] The mechanisms of virulence and immunity are often governed by molecular interactions between pathogens and host proteins. The study of these interactions has major implications on understanding virulence activities, and how the host immune system recognizes the presence of pathogens to initiate an immune response. Frequently, the association ...

Accelerated Snowmelt Protocol to Simulate Climate Change Induced Impacts on Snowpack Dependent Ecosystems

Authors: Laura T. Leonard, Chelsea Wilmer, Heidi Steltzer, Kenneth H. Williams and Jonathan O. Sharp, date: 03/20/2020, view: 2885, Q&A: 0

[Abstract] Field studies that simulate the effects of climate change are important for a predictive understanding of ecosystem responses to a changing environment. Among many concerns, regional warming can result in advanced timing of spring snowmelt in snowpack dependent ecosystems, which could lead to longer snow-free periods and drier summer soils. Past ...

High-resolution and Deep Phylogenetic Reconstruction of Ancestral States from Large Transcriptomic Data Sets

Authors: Sumanth Kumar Mutte and Dolf Weijers, date: 03/20/2020, view: 3739, Q&A: 0

[Abstract] Phylogenetics is an important area of evolutionary biology that helps to understand the origin and divergence of genes, genomes and species. Building meaningful phylogenetic trees is needed for the accurate reconstruction of the past. To achieve a correct phylogenetic understanding of genes or proteins, reliable and robust methods are needed to ...

Quantification of Blumenol Derivatives as Leaf Biomarkers for Plant-AMF Association

Authors: Elisabeth Mindt, Ming Wang, Martin Schäfer, Rayko Halitschke and Ian T. Baldwin, date: 07/20/2019, view: 4487, Q&A: 0

[Abstract] Symbiotic interactions between arbuscular mycorrhizal fungi (AMF) and plants are widespread among land plants and can be beneficial for both partners. The plant is provided with mineral nutrients such as nitrogen and phosphorous, whereas it provides carbon resources for the fungus in return. Due to the large economic and environmental impact, ...

Determination of Root Exudate Concentration in the Rhizosphere Using ¹³C Labeling

Authors: Laurent Simon and Feth el Zahar Haichar, date: 05/05/2019, view: 4927, Q&A: 0

[Abstract] One of the most remarkable metabolic features of plant roots is their ability to secrete a wide range of compounds into the rhizosphere, defined as the volume of soil around living roots. Around 5%-21% of total photosynthetically fixed carbon is transferred into the rhizosphere through root exudates. Until recently, studies on the quantity and ...

Measuring and Imaging the Soil-root-water System with a Light Transmission 2D Technique

Authors: Claude Doussan and Emmanuelle Garrigues, date: 03/20/2019, view: 5155, Q&A: 0

[Abstract] Improving crops against water deficits requires a better understanding of plant root system functioning. This requires a better knowledge of the water uptake process and to address the influence of root system architecture or root physiological properties on the uptake efficiency. To this end, we describe here a non-destructive system that enables ...

GC-MS-Based Analysis of Methanol: Chloroform-extracted Fatty Acids from Plant Tissues

Authors: Manish Kumar Patel, Shubhashis Das and Jitendra Kumar Thakur, date: 09/20/2018, view: 11666, Q&A: 0

[Abstract] Fatty acids (FAs) are carboxylic acids with long aliphatic chains that may be straight, branched and saturated or unsaturated. Most of the naturally occurring plant FAs contains an even number of carbon (C4-C24). FAs are used in food and pharmacological industries due to their nutritional importance. In addition, FAs are considered as a promising ...

An Inexpensive and Comprehensive Method to Examine and Quantify Field Insect Community Influenced by Host Plant Olfactory Cues

Authors: Rupesh Kariyat, Jesus Chavana and Jasleen Kaur, date: 08/20/2018, view: 4629, Q&A: 0

[Abstract] Insect pollinators, herbivores and their natural enemies use olfactory cues emitted by their host plants to locate them. In insect-plant ecology, understanding the mechanisms underlying these interactions are of critical importance, as this bio-communication has both ecological and agricultural applications. However, the first step in such ...

In situ Hybridization (ISH) in Preparasitic and Parasitic Stages of the Plant-parasitic Nematode Meloidogyne spp.

Authors: Maëlle Jaouannet, Chinh-Nghia Nguyen, Michaël Quentin, Stéphanie Jaubert-Possamai, Marie-Noëlle Rosso and Bruno Favery, date: 03/20/2018, view: 7615, Q&A: 1

[Abstract] The spatio-temporal expression pattern of a gene provides important indications to better understand its biological function. In situ hybridization (ISH) uses a labeled complementary single-stranded RNA or DNA probe to localize gene transcripts in a whole organism, a whole organ or a section of tissue. We adapted the ISH technique to the ...

Plants

Protocol for Isolation of Cardiomyocyte from Adult Mouse and Rat

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