Drug Discovery

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    Protocols in Current Issue
    A Cell-based Screening Method Using an Intracellular Antibody for Discovering Small Molecules Targeting Hard-to-drug Proteins
    Authors:  Nicolas Bery and Terry H. Rabbitts, date: 02/20/2022, view: 1847, Q&A: 0
    [Abstract] Targeting hard-to-drug proteins, such as proteins functioning by protein-protein interactions (PPIs) with small molecules, is difficult because of the lack of well-defined pockets. Fragment or computational-based methods are usually employed for the discovery of such compounds, but no generic method is available to quickly identify small molecules ...
    Amber Suppression Technology for Mapping Site-specific Viral-host Protein Interactions in Mammalian Cells
    Authors:  Nur Firdaus Isa, Olivier Bensaude and Shona Murphy, date: 02/05/2022, view: 1112, Q&A: 0
    [Abstract]

    Probing the molecular interactions of viral-host protein complexes to understand pathogenicity is essential in modern virology to help the development of antiviral therapies. Common binding assays, such as co-immunoprecipitation or pull-downs, are helpful in investigating intricate viral-host proteins interactions. However, such assays may miss

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    Rapid in vitro and in vivo Evaluation of Antimicrobial Formulations Using Bioluminescent Pathogenic Bacteria
    Authors:  Artur Schmidtchen and Manoj Puthia, date: 01/20/2022, view: 1581, Q&A: 0
    [Abstract]

    Basic and translational research needs rapid methods to test antimicrobial formulations. Bioluminescent bacteria and advanced imaging systems capable of acquiring bioluminescence enable us to quickly and longitudinally evaluate the efficacy of antimicrobials. Conventional approaches, such as radial diffusion and viable count assays, are

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