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Protocol for Isolation of Cardiomyocyte from Adult Mouse and Rat

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Isolation of Murine Primary Aortic Smooth Muscle Cells

Authors: Max Ole Hubert, Juan Rodriguez-Vita, Lena Wiedmann and Andreas Fischer, date: 02/05/2021, view: 3380, Q&A: 1

[Abstract]

Vascular smooth muscle cells (VSMCs) have been cultured for decades to study the role of these cells in cardiovascular disorders. The most common source of VSMCs is the rat aorta. Here we show the adaptation of this method to isolate and culture mouse aortic VSMCs. The advantage of this method is that there are many more transgenic mouse lines

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Imaging the Vasculature of Immunodeficient Mice Using Positron Emission Tomography/Computed Tomography (PET/CT) and 18F-fluorodeoxyglucose Labeled Human Erythrocytes

Authors: Shaowei Wang and Jung W. Choi, date: 10/05/2019, view: 3283, Q&A: 0

[Abstract] Nuclear blood pool imaging using radiolabeled red blood cells has been used in the clinical setting for the evaluation of a number of medical conditions including gastrointestinal hemorrhage, impaired cardiac contractility, and altered cerebrovascular blood flow. Nuclear blood pool imaging is typically performed using Technetium-99m-labeled (^99m ...

Human Endothelial Cell Spheroid-based Sprouting Angiogenesis Assay in Collagen

Authors: Fabian Tetzlaff and Andreas Fischer, date: 09/05/2018, view: 14515, Q&A: 1

[Abstract] Angiogenesis, the formation of new blood vessels from pre-existing ones plays an important role during organ development, regeneration and tumor progression. The spheroid-based sprouting assay is a well-established and robust method to study the influence of genetic alterations or pharmacological compounds on capillary-like tube formation of ...

Evaluation of Angiogenesis Inhibitors Using the HUVEC Fibrin Bead Sprouting Assay

Authors: Laura Winters, Nithya Thambi, Julian Andreev and Frank Kuhnert, date: 10/05/2016, view: 12265, Q&A: 0

[Abstract] Angiogenesis, the growth of new blood vessels from pre-existing vessels, is a critical process that occurs during normal development and tumor formation. Targeting tumor angiogenesis by blocking the activity of vascular endothelial growth factor (VEGF) has demonstrated some clinical benefit; nevertheless there is a great need to target additional ...

Rat Aortic Ring Model to Assay Angiogenesis ex vivo

Authors: Isabelle Ernens, Bénédicte Lenoir, Yvan Devaux and Daniel R. Wagner, date: 10/20/2015, view: 9570, Q&A: 0

[Abstract] Angiogenesis is a multifactorial event which requires the migration, proliferation, differentiation and structure rearrangement of endothelial cells. This angiogenic process has been commonly studied using in vitro assays such as Boyden chamber assay, wound healing assay and tube formation assay. These assays mainly use monolayers of ...

In vivo Chick Chorioallantoic Membrane (CAM) Angiogenesis Assays

Authors: Zhenguo Chen, Zhihua Wen and Xiaochun Bai, date: 09/20/2013, view: 35557, Q&A: 3

[Abstract] Angiogenesis is the process of formation of new blood vessels from pre-existing vessels or endothelial cell progenitors. It plays a crucial role in tumor growth and metastasis. Tumor angiogenesis have been widely studied as an important target for suppressing tumor growth and metastasis. Here, we describe an in vivo chick embryo ...

In vitro Human Umbilical Vein Endothelial Cells (HUVEC) Tube-formation Assay

Authors: Josephine MY Ko and Maria Li Lung, date: 09/20/2012, view: 63230, Q&A: 1

[Abstract] Angiogenesis is involved not only in pathological conditions including cancer biology and non-neoplastic diseases, but also many biological processes including reproduction, development and repair. During angiogenesis, endothelial cells (ECs) undergo activation after binding of angiogenic factors to their receptors, release of proteases to ...

In vivo Matrigel Plug Angiogenesis Assay

Authors: Hong Lok Lung and Maria Li Lung, date: 09/20/2012, view: 48091, Q&A: 4

[Abstract] The matrigel plug angiogenesis assay is a simple in vivo technique to detect the newly formed blood vessels in the transplanted gel plugs in nude mice. The matrigel matrix is derived from the engelbroth-holm-swarm (EHS) mouse sarcoma, and its composition is comparable to the basement membrane proteins. The matrigel can induce ...

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