Microbiology

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    Different Methods of Soil DNA Extraction
    Authors:  Asmita Kamble and Harinder Singh, date: 01/20/2020, view: 2631, Q&A: 0
    Soil is the major reservoir of microbial diversity. Only 1% of microbial diversity can be cultured while 99% is still not culturable. It is necessary to extract DNA from soil in order to explore the 99% microbial diversity, which will be useful to ...
    Isolation of PCR-quality Genomic DNA from Soils Impacted with Extra Heavy Crude Oil
    For the study of microbial communities in samples of soils impacted with extra heavy crude oil, it is necessary to perform molecular analyses. Due to the difficulty of oil matrix handling, there are very few protocols reported in writing. Also, one ...
    E. coli Genomic DNA Extraction
    Author:  Fanglian He, date: 07/20/2011, view: 192834, Q&A: 53
    This protocol uses phenol/chloroform method to purify genomic DNA without using commercial kits.
    Yeast Transcription Factor Chromatin Immunoprecipitation
    Author:  Wei Zheng, date: 07/05/2011, view: 12105, Q&A: 0
    This ChIP protocol was developed and improved over the years by various researchers in the Snyder lab, Stanford University, especially Anthony Borneman and Christopher Yellman. I have used this method to successfully map the genome-wide binding of ...
    Yeast Genomic DNA Miniprep Using A FastPrep Cell Lyser
    Author:  Xiyan Li, date: 05/05/2011, view: 10646, Q&A: 3
    This method is a convenient way to purify high-quality genomic DNA from yeast cells. It is suitable for PCR and other assays that require genomic DNA of higher quality.
    Plasmid DNA Extraction from E. coli Using Alkaline Lysis Method
    Author:  Fanglian He, date: 02/05/2011, view: 193430, Q&A: 48
    This is a quick and efficient way to extract E. coli plasmid DNA without using commercial kits. This technique was invented by Birnboim and Doly (1979).
    Colony PCR Using Yeast Spheroplasted Cells
    Author:  Bio-protocol Editor, date: 01/05/2011, view: 12699, Q&A: 1
    The first part of this protocol involves the removal of yeast cell walls using the Zymolase enzyme. The resulting spheroplast cells can then be used as template for PCR. This quick and easy to implement protocol describes how to prepare ...



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