Plant Science

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    Preparing Immunolocalization Slides of Maize Meiotic Chromosomes for Three-dimensional Microscopy
    Authors:  Qian Du and Wojciech P. Pawlowski, date: 07/20/2020, view: 123, Q&A: 0
    The protocol provides fully detailed steps for preparing microscopic slides of acrylamide-embedded maize meiotic cells. This method is particularly useful for examining chromatin structure and chromosome arrangement without destroying the ...
    Chiasma Counting in Maize Male Meiocytes
    Authors:  Gaganpreet K. Sidhu and Wojciech P. Pawlowski, date: 05/05/2020, view: 457, Q&A: 0
    This protocol combines a classical chromosome spreading technique with 3-dimensional image reconstruction for visualization of bivalent configurations during meiosis to aid chiasma counting.
    Immunolabeling of Maize Meiocytes
    Authors:  Mateusz Zelkowski and Wojciech P. Pawlowski, date: 04/05/2020, view: 550, Q&A: 0
    This protocol describes a quick method for immunolocalization of meiotic proteins in maize. It includes a fixation step that allows for long-term storage of material and provides good preservation of chromatin structure.



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