Plant Science

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Western Blot in Maize

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    Western Blot in Maize
    Author:  María Jazmín Abraham-Juárez, date: 06/05/2019, view: 466, Q&A: 0
    Since its introduction in 1979 (Towbin et al., 1979), protein blotting (Western blot) has become a routine tool in molecular biology research. It is used to detect low amounts of proteins in complex samples or to monitor protein expression ...
    Maize Protein Extraction for Different Downstream Applications
    Author:  María Jazmín Abraham-Juárez, date: 05/20/2019, view: 299, Q&A: 0
    Sample preparation is a key step for making plant proteomics analyses reproducible. The large amount of organic compounds, complex polysaccharides, proteases and other cellular components in vascular plants interfere with the analysis of proteins ...
    Extraction of Root Apoplastic Wall Fluid for Apoplastic Peroxidase Activity Assay
    Author:  Xiyan Li, date: 09/05/2011, view: 12643, Q&A: 0
    Plant roots secret a lot of peroxidases to counteract environmental influences. This protocol describes a way to extract root apoplastic wall fluid from Arabidopsis plants and to determine the peroxidase activity using guaiacol as substrate.