Cell Biology

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Western Blot in Maize

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    A Blood-retina Barrier Permeability Assay in Young Mice Using Sulfo-NHS-LC-biotin Perfusion
    Authors:  Entzu Wan, Mitsutaka Ogawa and Tetsuya Okajima, date: 10/20/2018, view: 1309, Q&A: 0
    Brain and retinal vasculatures exhibit restricted vascular permeability known as blood-brain barrier and blood-retina barrier. Vascular permeability can be evaluated by perfusion of the amine reactive ester derivatives of biotin such as ...
    Oil Red O Staining of Fixed Worm
    Author:  Fanglian He, date: 07/05/2012, view: 20915, Q&A: 1
    Oil red O staining is used to assess major fat stores in C. elegans. This protocol is adapted from the Ashrafi Lab at the University of California-San Francisco.
    Preparation of Torula Yeast RNA for Hybe Solutions
    Author:  Lili Jing, date: 02/05/2012, view: 16615, Q&A: 5
    In situ hybridization in zebrafish embryos frequently suffers from high background signal. Torular yeast RNA is often added to reduce the non-specific binding that leads to the high background signal. Without appropriate preparation, ...
    In situ Hybridization in Zebrafish Embryos
    Author:  Lili Jing, date: 02/05/2012, view: 12212, Q&A: 0
    In situ hybridization is routinely used to examine the gene expression level and location of embryos. This protocol is modified from the Thisse protocol and is a detailed description of the in situ hybridization procedures in ...
    Double in situ Hybridization
    Author:  Lili Jing, date: 02/05/2012, view: 13269, Q&A: 0
    Double in situ hybridization I very useful to examine the relationship between the expression of two genes. But it is tricky because of the cross reaction of two different antibodies. This protocol is a valid method to do double color in ...
    pH3 Antibody Staining Protocol for Zebrafish
    Author:  Lili Jing, date: 01/20/2012, view: 21992, Q&A: 0
    Phosphorylation of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Staining for phosphor histone H3 is a good indicator for the proliferation of the interested cells. This protocol provides a method to ...
    Alarmablue Assay for Detecting Cell Viability
    Author:  Huagang Zhang, date: 08/20/2011, view: 12010, Q&A: 0
    AlamarBlueTM detects cell viability by utilizing a nonfluorescent dye resazurin, which is converted to a fluorescent dye resorufin in response to chemical reduction of growth medium resulting from cell growth. The fluorescent or ...
    DAPI Nuclear Staining of Live Worm
    Author:  Fanglian He, date: 06/05/2011, view: 15844, Q&A: 0
    Adapted from the Villenueve Lab at Stanford University. This is a very simple method using ethanol fixation, but works very well.
    Yeast Vacuole Staining with FM4-64
    Author:  Zongtian Tong, date: 01/05/2011, view: 23290, Q&A: 2
    The lipophilic probe, FM 4-64 does not fluoresce much in water but fluoresces strongly after binding to the outer plasma membrane, providing clear and distinguishable plasma membrane staining. The binding is rapid and reversible. In this protocol ...