Plant Science

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    Chromatographic Analysis for Targeted Metabolomics of Antioxidant and Flavor-Related Metabolites in Tomato
    Authors:  Nergiz Gürbüz Çolak, Neslihan Tek Eken, Anne Frary and Sami Doğanlar, date: 03/05/2021, view: 1246, Q&A: 0

    Targeted metabolomics is a useful approach to evaluate crop breeding studies. Antioxidant and flavor-related traits are of increasing interest and are considered quality traits in tomato breeding. The present study presents chromatographic methods

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    Gene Expression and Genome Editing Systems by Direct Delivery of Macromolecules Into Rice Egg Cells and Zygotes
    Authors:  Erika Toda and Takashi Okamoto, date: 07/20/2020, view: 1437, Q&A: 0
    Polyethylene glycol calcium (PEG-Ca2+)-mediated transfection allows rapid and efficient examination to analyze diverse cellular functions of genes of interest. In plant cells, macromolecules, such as DNA, RNA and protein, are delivered ...
    In vitro Conditions for Dark Growth and Analysis of Maize Seedlings
    Authors:  Jeffrey Farrow, Marschal A. Bellinger and Carolyn G. Rasmussen, date: 03/20/2020, view: 1622, Q&A: 0
    Maize is an important model organism for understanding plant traits essential for proper growth and germination. One type of growth, skotomorphogenesis, occurs in the absence of light. Seedlings grown in the absence of light exhibit dramatic ...
    Using Seed Chipping to Genotype Maize Kernels
    Authors:  Alison M. Mills, Lindy A. Allsman, Sareen Leon and Carolyn G. Rasmussen, date: 03/20/2020, view: 2180, Q&A: 0
    Zea mays (maize) is an important model organism for studying monocot growth and development. Genotyping maize in the greenhouse or field can be time consuming and costly. Here, we describe a method to remove or chip a small amount of the ...
    Mating and Tetrad Dissection in Chlamydomonas
    Author:  Frej Tulin, date: 04/05/2019, view: 3287, Q&A: 0
    The ability to carry out controlled genetic crosses has contributed to making the unicellular green alga Chlamydomonas reinhardtii an important model organism for the green plant kingdom (Merchant et al., 2007). This protocol ...
    Micropropagation of Prickly Pear by Axillary Shoot Proliferation
    A protocol for the axillary bud proliferation of prickly pear (Opuntia; Cactaceae) is presented. This genus is widely used as a crop in the arid and semi-arid areas of the globe worldwide, providing numerous benefits for human and animal ...



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