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A high-quality database for basic life science protocols
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A high-quality database for basic life science protocols
Biochemistry
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Western Blot in Maize
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Modifications in the Transformation Step of Commercially Available Site-directed Mutagenesis Kit Increase Its Price-performance
Authors:
Atta Ahmad
,
David F. Jahad
,
Hamzah Karimi
,
Mary A. Farwell
,
Jean-Luc Scemama
and
Cindy Putnam-Evans
,
date:
08/05/2019,
view:
652,
Q&A:
0
Site-directed mutagenesis helps unravel the relationship between the structure and function of a protein at the level of amino acid contributions. Many techniques have been established to carry out site-directed mutagenesis on a daily routine. ...
Western Blot in Maize
Author:
María Jazmín Abraham-Juárez
,
date:
06/05/2019,
view:
1949,
Q&A:
0
Since its introduction in 1979 (Towbin
et al.
, 1979), protein blotting (Western blot) has become a routine tool in molecular biology research. It is used to detect low amounts of proteins in complex samples or to monitor protein expression ...
Maize Protein Extraction for Different Downstream Applications
Author:
María Jazmín Abraham-Juárez
,
date:
05/20/2019,
view:
1074,
Q&A:
0
Sample preparation is a key step for making plant proteomics analyses reproducible. The large amount of organic compounds, complex polysaccharides, proteases and other cellular components in vascular plants interfere with the analysis of proteins ...
Very Low Molecular Weight Proteins Electrophoresis Protocol
Authors:
César E. Rivera
,
José D. Rosales
,
Juan C. Freites-Perez
and
Eliaira Rodriguez
,
date:
11/20/2018,
view:
4322,
Q&A:
1
The electrophoresis is the most used technique to separate proteins and usually the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) proposed by Laemmli is the prefered since it can diferentiate large size proteins, but for very ...
SDS-PAGE for Silk Fibroin Protein
Author:
Yu-Qing Zhang
,
date:
10/20/2018,
view:
3931,
Q&A:
0
The method and detailed procedure of SDS-PAGE for silk proteins are exactly the same as for other proteins, but the electrophoresis profile of silk protein is often unsatisfactory. The main reason is that their molecular masses are too large, and ...
A Lentiviral Pseudotype ELLA for the Measurement of Antibodies Against Influenza Neuraminidase
Authors:
Fabrizio Biuso
,
George Carnell
,
Emanuele Montomoli
and
Nigel Temperton
,
date:
07/20/2018,
view:
2726,
Q&A:
0
This protocol describes the rapid and safe production of lentiviral pseudotypes characterized by a lentiviral core containing a reporter, in conjunction with avian influenza haemagglutinin (HA) and human neuraminidase (NA) glycoproteins on the ...
In vitro
Chaperone Activity Assay Using α-Amylase as Target Protein
Authors:
Jeeshma Nambidi Parambath
,
Gayathri Valsala
,
Karthik Menon
and
Shiburaj Sugathan
,
date:
06/20/2018,
view:
3341,
Q&A:
0
Small heat shock proteins (sHSP) are stress proteins which are ubiquitously found in almost all living organisms. They function as molecular chaperones, which assist in protein folding during translation and in the prevention of irreversible protein ...
Purifying Properly Folded Cysteine-rich, Zinc Finger Containing Recombinant Proteins for Structural Drug Targeting Studies: the CH1 Domain of p300 as a Case Example
Authors:
Yong Joon Kim
,
Stefan Kaluz
,
Anil Mehta
,
Emily Weinert
,
Shannon Rivera
and
Erwin G. Van Meir
,
date:
09/05/2017,
view:
7244,
Q&A:
0
The transcription factor Hypoxia-Inducible Factor (HIF) complexes with the coactivator p300, activating the hypoxia response pathway and allowing tumors to grow. The CH1 and CAD domains of each respective protein form the interface between p300 and ...
Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-histone Antibodies
Author:
Zheng Liu
,
date:
06/20/2012,
view:
9529,
Q&A:
0
Autoimmune disease is characterized with the break of tolerance against self antigens. Anti-histone antibodies can be found in the majority of patients with system lupus erythematosus (SLE) and a number of lupus-prone mouse strains. This protocol ...
RNP-IP (Original Method): Obtaining Majority RNA from RNA Binding Protein in the Nucleus
Author:
FengZhi Liu
,
date:
05/20/2012,
view:
16895,
Q&A:
1
Post-transcriptional regulation of gene expression is a ribonucleoprotein (RNP)-driven process, which involves RNA binding proteins (RBPs) and noncoding RNAs that regulate splicing, nuclear export, subcellular localization, mRNA stability and ...