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A high-quality database for basic life science protocols
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A high-quality database for basic life science protocols
Cell Biology
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Tail Tendon Break Time for the Assessment of Aging and Longitudinal Healthspan in Mice
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MTT Assay for Cytotoxicity Assessment in
Oryza sativa
Root Tissue
Authors:
Snehalata Majumdar
,
Titir Guha
and
Rita Kundu
,
date:
11/20/2017,
view:
6742,
Q&A:
0
Cytotoxicity of different compounds are commonly evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. This assay is mainly used to study cell viability in cell lines (Carmichael
et al.
, 1987). In this ...
Lifespan Assay
Author:
Fanglian He
,
date:
04/05/2011,
view:
25515,
Q&A:
3
This assay is used to address aging-related questions in worms.
MTT Assay of Cell Numbers after Drug/Toxin Treatment
Author:
Ran Chen
,
date:
04/05/2011,
view:
22483,
Q&A:
1
MTT assay is a colorimetric method for measuring the activity of enzymes in living cells that reduce MTT to formazan dyes, giving a purple color. It is commonly used to determine cytotoxicity of potential medicinal agents and toxic materials, since ...
Protocol for Whole Cell Lysis of Yeast
Author:
Bio-protocol Editor
,
date:
01/05/2011,
view:
38970,
Q&A:
5
This protocol describes how to perform lysis on whole yeast cell samples using NaOH. The lysed cells can then be used for downstream applications such as the extraction of total proteins.
Metabolic Labeling of Yeast Proteins
Author:
Bio-protocol Editor
,
date:
01/05/2011,
view:
12326,
Q&A:
0
Proteins of
Saccharomyces cerevisiae
can be metabolically labeled with (35) methionine. After labeling, a protocol is described for the mechanical disruption of yeast cells or conversion to spheroplasts, with subsequent lysis before ...
How to Use an Avestin Emulsiflex C3 Homogenizer to Disrupt Cells
Author:
Bio-protocol Editor
,
date:
01/05/2011,
view:
20957,
Q&A:
1
The EmulsiFlex-C3 homogenizer is powered by an electric motor. The pump does not require a compressor for it to run. This equipment can be used to disrupt cells at a large scale. The EmulsiFlex-C3 has a fixed flow-through capacity of 3 L/h. It has ...
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