Cell Biology

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Western Blot in Maize

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    MTT Assay for Cytotoxicity Assessment in Oryza sativa Root Tissue
    Authors:  Snehalata Majumdar, Titir Guha and Rita Kundu, date: 11/20/2017, view: 4341, Q&A: 0
    Cytotoxicity of different compounds are commonly evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. This assay is mainly used to study cell viability in cell lines (Carmichael et al., 1987). In this ...
    Lifespan Assay
    Author:  Fanglian He, date: 04/05/2011, view: 22181, Q&A: 3
    This assay is used to address aging-related questions in worms.
    MTT Assay of Cell Numbers after Drug/Toxin Treatment
    Author:  Ran Chen, date: 04/05/2011, view: 19522, Q&A: 1
    MTT assay is a colorimetric method for measuring the activity of enzymes in living cells that reduce MTT to formazan dyes, giving a purple color. It is commonly used to determine cytotoxicity of potential medicinal agents and toxic materials, since ...
    Protocol for Whole Cell Lysis of Yeast
    Author:  Zongtian Tong, date: 01/05/2011, view: 32584, Q&A: 5
    This protocol describes how to perform lysis on whole yeast cell samples using NaOH. The lysed cells can then be used for downstream applications such as the extraction of total proteins.
    Metabolic Labeling of Yeast Proteins
    Author:  Zongtian Tong, date: 01/05/2011, view: 10090, Q&A: 0
    Proteins of Saccharomyces cerevisiae can be metabolically labeled with (35) methionine. After labeling, a protocol is described for the mechanical disruption of yeast cells or conversion to spheroplasts, with subsequent lysis before ...
    How to Use an Avestin Emulsiflex C3 Homogenizer to Disrupt Cells
    Author:  Zongtian Tong, date: 01/05/2011, view: 17801, Q&A: 1
    The EmulsiFlex-C3 homogenizer is powered by an electric motor. The pump does not require a compressor for it to run. This equipment can be used to disrupt cells at a large scale. The EmulsiFlex-C3 has a fixed flow-through capacity of 3 L/h. It has ...