Neuroscience

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    Clearing of the Mouse Brain for Optical Imaging Using CUBIC
    Authors:  Lianxiang Luo, Xiaoyu Chen, Ruijian Wu, Han Wang and Hui Luo, date: 08/20/2021, view: 204, Q&A: 0

    Here, we describe a simple and efficient tissue clearing method, CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis), which was first introduced in 2014 (Susaki et al., 2014). In the present study, the hemispheres of

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    Optical Clearing and 3D Analysis Optimized for Mouse and Human Pancreata

    The pancreas is a heavily innervated organ, but pancreatic innervation can be challenging to comprehensively assess using conventional histological methods. However, recent advances in whole-mount tissue clearing and 3D rendering techniques have

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    Identification of Socially-activated Neurons
    Authors:  Mary L. Phillips and Lucas Pozzo-Miller, date: 09/05/2020, view: 3069, Q&A: 0
    Determining the neuronal circuitry responsible for specific behaviors is a major focus in the field of neurobiology. Activity-dependent immediate early genes (IEGs), transcribed and translated shortly after neurons discharge action potentials, have ...
    Examining Cocaine Conditioning Place Preference in Mice
    Authors:  MaryElizabeth J. Simkevich, Rianne R. Campbell and André O. White, date: 04/20/2020, view: 2172, Q&A: 0
    A key component of combating substance use disorders is understanding the neural mechanisms that support drug reward. Tasks such as self-administration assess the reinforcing properties of a drug using a learned behavior but require numerous ...
    The Mouse Gambling Task: Assessing Individual Decision-making Strategies in Mice
    Authors:  Elsa Pittaras, Arnaud Rabat and Sylvie Granon, date: 01/05/2020, view: 2535, Q&A: 0
    Decision-making is a complex cognitive process which consists of choosing one option among several alternatives. In humans, this process is featured in the Iowa gambling task (IGT), a decision-making task that mimics real life situations by ...
    Construction of Viral Vectors for Cell Type-specific CRISPR Gene Editing in the Adult Mouse Brain
    Authors:  Hiroshi Yamaguchi and Luis de Lecea, date: 08/20/2019, view: 3522, Q&A: 0
    Recently developed gene editing technologies based on engineered CRISPR/Cas9 systems enables researchers to disrupt genes in a cell type-specific manner in the adult mouse brain. Using these technologies, we recently showed that the dopamine ...
    Looking through Brains with Fast Passive CLARITY: Zebrafish, Rodents, Non-human Primates and Humans
    Authors:  Farzad Mortazavi, Alexander J. Stankiewicz and Irina V. Zhdanova, date: 08/05/2019, view: 5614, Q&A: 0
    Recently developed CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ-hybridization-compatible Tis-sue-hYdrogel) technique renders the tissue transparent by removing lipids in the tissue, while preserving and ...
    Accessing Olfactory Habituation in Drosophila melanogaster with a T-maze Paradigm
    Authors:  Ourania Semelidou, Summer F. Acevedo and Efthimios M.C. Skoulakis, date: 06/05/2019, view: 3933, Q&A: 0
    Habituation is the process whereby perceptual changes alter the value of environmental stimuli, enabling salience filtering. This behavioral response decrement is a form of non-associative learning, where the subject learns about the stimulus and ...
    High-resolution Immunoelectron Microscopy Techniques for Revealing Distinct Subcellular Type 1 Cannabinoid Receptor Domains in Brain
    Activation of type 1 cannabinoid (CB1) receptors by endogenous, exogenous (cannabis derivatives) or synthetic cannabinoids (i.e., CP 55.940, Win-2) has a wide variety of behavioral effects due to the presence of CB1 ...
    Optical Clearing and Index Matching of Tissue Samples for High-resolution Fluorescence Imaging Using SeeDB2
    Authors:  Meng-Tsen Ke and Takeshi Imai, date: 10/20/2018, view: 7971, Q&A: 1
    Tissue clearing techniques are useful for large-scale three-dimensional fluorescence imaging of thick tissues. However, high-resolution imaging deep inside tissues has been challenging, as it is extremely sensitive to light scattering and spherical ...



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