Plant Science

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    Staining Maize Epidermal Leaf Peels with Toluidine Blue O
    Authors:  Marschal A. Bellinger, Sukhmani K. Sidhu and Carolyn G. Rasmussen, date: 04/20/2019, view: 159, Q&A: 0
    Maize is an important model organism for understanding cell patterning and development. The regular patterning of maize leaf epidermal cells has previously been characterized using the dye toluidine blue O (TBO). The polychromatic dye TBO ...
    Glue Impressions of Maize Leaves and Their Use in Classifying Mutants
    Authors:  Lindy A. Allsman, Raquel N. Dieffenbacher and Carolyn G. Rasmussen, date: 04/05/2019, view: 577, Q&A: 0
    Maize cells are surrounded by cell walls that can be easily visualized using a simple protocol outlined below. A glue impression is an imprint of maize epidermal cells on a glass slide made using a cyanoacrylate-based glue (super glue) that produces ...
    Mating and Tetrad Dissection in Chlamydomonas
    Author:  Frej Tulin, date: 04/05/2019, view: 249, Q&A: 0
    The ability to carry out controlled genetic crosses has contributed to making the unicellular green alga Chlamydomonas reinhardtii an important model organism for the green plant kingdom (Merchant et al., 2007). This protocol ...
    Microscopic Observation of Subcellular GFP-tagged Protein Localization in Rice Anthers
    Authors:  Seijiro Ono and Ken-Ichi Nonomura, date: 08/05/2018, view: 1302, Q&A: 0
    This protocol demonstrates a simple method to determine the subcellular localization of fluorescence-tagged proteins on the vibratome sections of rice developing anthers. If a cell type-specific promoter is used to drive the tagged protein-encoding ...
    Visualization of Growth and Morphology of Fungal Hyphae in planta Using WGA-AF488 and Propidium Iodide Co-staining
    Authors:  Amey Redkar, Elaine Jaeger and Gunther Doehlemann, date: 07/20/2018, view: 1937, Q&A: 0
    Fungal pathogens colonizing plants show a varying degree of symptoms. Microscopy techniques have been used to study the infection and proliferation of fungal hyphae inside the host. One of the best optimized and commonly used method is the ...
    Micropropagation of Prickly Pear by Axillary Shoot Proliferation
    A protocol for the axillary bud proliferation of prickly pear (Opuntia; Cactaceae) is presented. This genus is widely used as a crop in the arid and semi-arid areas of the globe worldwide, providing numerous benefits for human and animal ...
    MTT Assay for Cytotoxicity Assessment in Oryza sativa Root Tissue
    Authors:  Snehalata Majumdar, Titir Guha and Rita Kundu, date: 11/20/2017, view: 3885, Q&A: 0
    Cytotoxicity of different compounds are commonly evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. This assay is mainly used to study cell viability in cell lines (Carmichael et al., 1987). In this ...
    Leaf Clearing Protocol to Observe Stomata and Other Cells on Leaf Surface
    Author:  Nidhi Sharma, date: 09/05/2017, view: 7141, Q&A: 1
    In this protocol, leaves are cleared and fixed in an ethanol and acetic acid solution, and mounted in Hoyer’s solution. The cleared leaves are imaged under differential interference contrast (DIC) microscope. This protocol is beneficial for studying ...
    Extraction of Root Apoplastic Wall Fluid for Apoplastic Peroxidase Activity Assay
    Author:  Xiyan Li, date: 09/05/2011, view: 12216, Q&A: 0
    Plant roots secret a lot of peroxidases to counteract environmental influences. This protocol describes a way to extract root apoplastic wall fluid from Arabidopsis plants and to determine the peroxidase activity using guaiacol as substrate.
    Histostaining for Tissue Expression Pattern of Promoter-driven GUS Activity in Arabidopsis
    Author:  Xiyan Li, date: 07/05/2011, view: 22646, Q&A: 3
    Promoter-driven GUS (beta-glucuronidase) activity is the most commonly used technique for tissue-specific expression patterns in Arabidopsis. In this procedure, GUS enzyme converts 5-bromo-4-chloro-3-indolyl glucuronide (X-Gluc) to a blue product. ...