Immunology

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    Protein- and Sequencing-based Massively Parallel Single-cell Approaches to Gene Expression Profiling
    Authors:  Xuhuai Ji, Igor Goncharov, Miao Zhao, Molly Miranda and Holden T. Maecker, date: 02/05/2019, view: 3837, Q&A: 0
    Understanding cellular diversity and dynamics in immune cells are critical for elucidating mechanisms of diagnosis, response to therapeutics, and patient prognosis. Our goal is to apply a technically simple approach for gene expression cytometry ...
    Site Density Protocol
    Author:  Jun Huang, date: 07/05/2012, view: 6973, Q&A: 0
    The site densities of cell surface molecules are useful information for cell function analysis. Using antibody staining and commercial available calibration beads, this assay quantitatively determines the T cell receptor site density at the single T ...
    ELISA Measurement of Mouse IL-2
    Author:  Guo N. Huang, date: 01/05/2012, view: 9762, Q&A: 0
    Interleukin-2 (IL-2) is a cytokine secreted by T cells that is essential for immune system activation. This protocol is routinely used for quantification of IL-2 concentration in the supernant of cultured lymphocytes under various stimulations and ...
    In vitro Differentiation of Mouse Th0, Th1 and Th2 from Naïve CD4 T Cells
    Author:  Jia Li, date: 11/20/2011, view: 51167, Q&A: 20
    In vitro differentiation of helper T cells of various lineages is frequently used in T helper cell study. Naïve CD4 T cells can differentiate into certain lineage of T help cells in vitro in the presence of specific stimulatory ...
    Naïve T Lymphocyte Infection by Murine Stem Cell Virus (MSCV) Protocol
    Author:  Jia Li, date: 11/20/2011, view: 11064, Q&A: 0
    This assay can be used for studying genes related to lymphocyte proliferation and differentiation in vitro. Stimulating lymphocytes to proliferate is important for the infection efficiency of MSCV. After overnight culture, if naïve cells ...
    Thioglycollate Induced Peritonitis
    Author:  Zheng Liu, date: 06/20/2011, view: 29139, Q&A: 3
    Intraperitoneal (i.p.) injection of thioglycollate elicits a robust influx of neutrophils into peritoneal cavity. The trafficking of the cells is believed to be mediated by chemokines CXCL1, CXCL2, and CXCL8 (Call et al., 2001; Cacalano et ...
    In vivo BrdU Incorporation and Detection in Mouse
    Author:  Zheng Liu, date: 06/05/2011, view: 33305, Q&A: 0
    BrdU (Bromodeoxyuridine or 5-bromo-2’-deoxyuridine) is a synthetic nucleoside that is incorporated into DNA by proliferating cells. This protocol is to be used to incorporate and detect BrdU in murine plasma cells. The plasma cells described in this ...
    Immunofluorescent Staining of Murine Tissue
    Author:  Zheng Liu, date: 05/05/2011, view: 15327, Q&A: 0
    Renal immune complex deposition and leukocyte infiltration are characteristic of lupus nephritis in human patients and lupus-prone mice. This protocol describes how to stain frozen sections of murine kidney to study these features using fluorescent ...
    siRNA Transfection of Mouse Bone Marrow-derived Macrophages
    Author:  Ran Chen, date: 02/05/2011, view: 20709, Q&A: 2
    Short interfering RNAs (siRNA) are a type of double-stranded RNA molecule, typically 20-25 bp long, that are involved in the phenomenon of RNA interference. siRNA transfection is employed in this protocol to knockdown target gene expression in ...



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